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Clinical Chemistry 42: 1911-1914, 1996;
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Clinical Chemistry, Vol 42, 1911-1914, Copyright © 1996 by American Association for Clinical Chemistry

Highly sensitive ELISA for soluble Fas in serum: increased soluble Fas in the elderly

M Seishima, M Takemura, K Saito, H Sano, S Minatoguchi, H Fujiwara, T Hachiya and A Noma
Department of Laboratory Medicine, Gifu University School of Medicine, Japan. seishima@.cc.gifu-u.ac.jp

We have developed and characterized a highly sensitive ELISA for soluble Fas (sFas) in the serum. The linearity of calibrator range was 0.06-2.00 micrograms/L and the detection limit was 0.01 microgram/L. The average within- and between-run CVs were 3.9% and 3.8%, respectively. The recovery of added sFas to serum was 93-118%. The effects of possible interferences (tryglyceride, hemoglobin, bilirubin) were negligible. We determined serum sFas in 155 healthy subjects, ages 20-69. The mean value of sFas in men (2.50 +/- 0.63 micrograms/L, n = 78) was significantly higher than that in women (2.01 +/- 0.53 micrograms/L, n = 77) (P < 0.001). Furthermore, there was a significant correlation between serum sFas concentration and age (men, r = 0.397, P < 0.001; women, r = 0.569, P < 0.001). Although the concentrations of sFas tended to increase with aging, it remains to be clarified how Fas- mediated apoptosis relates to aging.


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