Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 42: 1961-1969, 1996;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jette, D. C.
Right arrow Articles by Suresh, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jette, D. C.
Right arrow Articles by Suresh, M. R.

Clinical Chemistry, Vol 42, 1961-1969, Copyright © 1996 by American Association for Clinical Chemistry

Epitope mapping of prostate-specific antigen with monoclonal antibodies

DC Jette, FT Kreutz, BA Malcolm, DS Wishart, AA Noujaim and MR Suresh
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Canada.

Prostate-specific antigen (PSA) is a widely used marker for screening and monitoring prostate cancer. We identified and characterized the epitopes of two anti-PSA monoclonal antibodies (mAbs) designated B80 and B87. The epitopes were initially mapped as nonoverlapping by developing a sandwich immunoassay to measure PSA with the two anti-PSA mAbs. The two antibodies do not cross-react with homologous pancreatic kallikrein, but recognize epitopes unique to PSA. B80 and B87 can recognize both free and complexed PSA and hence measure total PSA. Epitope scanning and bacteriophage peptide library affinity selection procedures were used to identify and locate an epitope on PSA. A possible epitope for B80 was identified as being located on or near PSA amino acid residues 50-58 (-GRH-SLFHP-). The epitope for B87 was likely on an exposed nonlinear conformational determinant, unique to PSA, and not masked by the binding of B80 or alpha 1-antichymotrypsin.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
J. Leinonen, P. Wu, and U.-H. Stenman
Epitope Mapping of Antibodies against Prostate-specific Antigen with Use of Peptide Libraries
Clin. Chem., December 1, 2002; 48(12): 2208 - 2216.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Michel, G. Deleage, J.-P. Charrier, J. Passagot, N. Battail-Poirot, G. Sibai, M. Jolivet, and C. Jolivet-Reynaud
Anti-Free Prostate-specific Antigen Monoclonal Antibody Epitopes Defined by Mimotopes and Molecular Modeling
Clin. Chem., May 1, 1999; 45(5): 638 - 650.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Geiser, D. Schultz, A. Le Cardinal, H. Voshol, and C. Garcia-Echeverria
Identification of the Human Melanoma-associated Chondroitin Sulfate Proteoglycan Antigen Epitope Recognized by the Antitumor Monoclonal Antibody 763.74 from a Peptide Phage Library
Cancer Res., February 1, 1999; 59(4): 905 - 910.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. T. Kreutz and M. R. Suresh
Novel bispecific immunoprobe for rapid and sensitive detection of prostate-specific antigen
Clin. Chem., April 1, 1997; 43(4): 649 - 656.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Association for Clinical Chemistry.