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Clinical Chemistry 42: 1996-2001, 1996;
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Clinical Chemistry, Vol 42, 1996-2001, Copyright © 1996 by American Association for Clinical Chemistry

High correlation but lack of agreement between direct high-performance gel chromatography analysis and conventional indirect methods for determining lipoprotein cholesterol

BR Krause, NJ Schork, KA Kieft, MP Smith and JJ Maciejko
Cardiac and Vascular Diseases, Parke-Davis Pharmaceutical Research Div., Warner-Lambert Co., Ann Arbor, MI 48105, USA. kraus01@aa.wl.com

Low-density lipoprotein-cholesterol (LDL-C) is currently estimated clinically by using the Friedewald formula, when plasma triglycerides are < 4000 mg/L, or as the difference between infranatant and high- density lipoprotein-cholesterol (HDL-C) values after ultracentrifugation of plasma at native density, when plasma triglycerides are > or = 4000 mg/L (beta quantification). HDL-C is measured by selective precipitation of apolipoprotein B-containing lipoproteins from whole plasma or from the density > 1.006 kg/L infranatant. We compared these conventional methods for LDL-C and HDL-C with "high-performance" gel chromatography (HPGC), a method that directly and simultaneously measures both LDL-C and HDL-C in a single, microliter volume of plasma. Not surprisingly, we found that the results by all these methods were highly correlated. However, LDL-C values were significantly higher and HDL-C values significantly lower by the direct HPGC method than by the conventional methods (paired t- test). In addition, both Bland-Altman plots and concordance correlation analyses indicated lack of agreement between the methods' results in the majority of patients' subgroups.


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 Clin. Chem.Home page
F. Gomez, J. Camps, J. M. Simo, N. Ferre, and J. Joven
Agreement Study of Methods Based on the Elimination Principle for the Measurement of LDL- and HDL-Cholesterol Compared with Ultracentrifugation in Patients with Liver Cirrhosis
Clin. Chem., August 1, 2000; 46(8): 1188 - 1191.
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J. M a Simo, I. Castellano, N. Ferre, J. Joven, and J. Camps
Evaluation of a homogeneous assay for high-density lipoprotein cholesterol: limitations in patients with cardiovascular, renal, and hepatic disorders
Clin. Chem., June 1, 1998; 44(6): 1233 - 1241.
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