Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 42: 2021-2027, 1996;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yaping, Z.
Right arrow Articles by Huiqin, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yaping, Z.
Right arrow Articles by Huiqin, C.

Clinical Chemistry, Vol 42, 2021-2027, Copyright © 1996 by American Association for Clinical Chemistry

Spectrophotometric determination of urinary iodine by flow-injection analysis with on-line catalytic digestion

Z Yaping, Y Dongxing, C Jixiang, L Tianshiu and C Huiqin
Longyan District Sanitation and Anti-Epidemic Station, P.R. China.

A flow-injection technique involving on-line catalytic digestion and spectrophotometric detection has been developed for the determination of iodine in urine. After urine samples are digested by KMnO4-K2Cr2O7- H2SO4 solution, the iodine in the urine catalyzes the reaction of As(III) with Ce(IV). The remaining Ce(IV) is then reacted with brucine and the product is detected with a spectrophotometer at 480 nm. With this technique, we obtained a detection limit for urinary iodine of 0.039 mumol/L, and the linear range was 0.039-7.88 mumol/L with a CV < 3%. Analytical recovery ranged between 92% and 104% (mean 99%). The sampling frequency of the flow-injection technique was 70/h. We applied the method to measure the iodine concentration in a freeze-dried urine reference sample and in collected urine samples, and compared the results with those obtained by the accepted alkaline ashing technique. The proposed technique has the advantages of being simple, rapid, precise, accurate, and sensitive. It can be used to assess iodine- deficient populations as well as those receiving treatment.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Association for Clinical Chemistry.