Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 42: 227-231, 1996;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jiang, Y. H.
Right arrow Articles by Chapkin, R. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, Y. H.
Right arrow Articles by Chapkin, R. S.

Clinical Chemistry, Vol 42, 227-231, Copyright © 1996 by American Association for Clinical Chemistry

Rapid competitive PCR determination of relative gene expression in limiting tissue samples

YH Jiang, LA Davidson, JR Lupton and RS Chapkin
Faculty of Nutrition, Molecular and Cell Biology Group, Texas A&M University, College Station, 77843-2471, USA.

Reverse transcriptase (RT)-PCR is widely used to study gene transcription in many biological systems. Despite the development of a variety of procedures, quantification of RT-PCR products remains difficult, particularly when processing a large number of samples. Therefore, we developed a novel alternative PCR technique that we term "rapid competitive PCR" (RC-PCR), designed to study the relative expression of specific genes in a large number of small tissue biopsies. RC-PCR is characterized by measuring relative gene expression at the mRNA level of two or more samples with a nonradioactive assay based on competitive PCR amplification between identical sequences of internal standard and target cDNA. Only a single reaction tube per sample is used in this technique, and it was validated by comparing RC- PCR of protein kinase C zeta and alpha expression in rat colonic mucosa samples with competitive RT-PCR analysis (requiring 6-8 reaction tubes per sample). We conclude that RC-PCR is a simple, rapid, highly sensitive technique that is capable of detecting less than twofold differences in mRNA expression.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Nutr.Home page
C. D. Davis and W. T. Johnson
Dietary Copper Affects Azoxymethane-Induced Intestinal Tumor Formation and Protein Kinase C Isozyme Protein and mRNA Expression in Colon of Rats
J. Nutr., May 1, 2002; 132(5): 1018 - 1025.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. S. Diamond, T. G. Roberts, D. Edgil, B. Lu, J. Ernst, and E. Harris
Modulation of Dengue Virus Infection in Human Cells by Alpha, Beta, and Gamma Interferons
J. Virol., June 1, 2000; 74(11): 4957 - 4966.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
M. V. Bor, B. S. Sorensen, P. Rammer, and E. Nexo
Calibrated user-friendly reverse transcriptase-PCR assay: quantitation of epidermal growth factor receptor mRNA
Clin. Chem., June 1, 1998; 44(6): 1154 - 1160.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
V. B. Thogersen, P. Bross, N. Gregersen, and E. Nexo
Quantitative Analysis of the Human Epidermal Growth Factor Receptor Messenger RNA Using Reverse Transcription-PCR: A Methodological Study of Imprecision
Clin. Chem., June 1, 1998; 44(6): 1344 - 1346.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Revillion, L. Hornez, and J.-P. Peyrat
Quantification of c-erbB-2 gene expression in breast cancer by competitive RT-PCR
Clin. Chem., November 1, 1997; 43(11): 2114 - 2120.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
Y.-H. Jiang, J. R. Lupton, and R. S. Chapkin
Dietary Fat and Fiber Modulate the Effect of Carcinogen on Colonic Protein Kinase C lambda  Expression in Rats
J. Nutr., October 1, 1997; 127(10): 1938 - 1943.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Association for Clinical Chemistry.