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Clinical Chemistry 42: 258-262, 1996;
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Clinical Chemistry, Vol 42, 258-262, Copyright © 1996 by American Association for Clinical Chemistry

Highly sensitive immunoradiometric assay for serum thyroglobulin with minimal interference from autoantibodies

PY Marquet, A Daver, R Sapin, B Bridgi, JP Muratet, DJ Hartmann, F Paolucci and B Pau
ERIA Diagnostics Pasteur, Marnes la Coquette, France.

Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated with four anti-Tg MAbs. After washing, the tubes are incubated with the tracer MAb for 20 h at room temperature. Dilution and reproducibility tests demonstrated assay reliability. Tests performed on samples with (n = 361) or without (n = 283) aAbs showed that the TG IRMA Pasteur is largely independent of the marked interference generally caused by aAbs. These results were confirmed with an extended population of 2759 samples. For a cutoff of 1 micrograms/L, sensitivity and specificity were 0.97 and 1, respectively, in a follow-up of differentiated thyroid carcinoma in patients treated by total thyroidectomy.


The following articles in journals at HighWire Press have cited this article:


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B. Lumbreras-Lacarra, J. M. Ramos-Rincon, and I. Hernandez-Aguado
Methodology in Diagnostic Laboratory Test Research in Clinical Chemistry and Clinical Chemistry and Laboratory Medicine
Clin. Chem., March 1, 2004; 50(3): 530 - 536.
[Abstract] [Full Text] [PDF]

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N. G. Morgenthaler, J. Froehlich, J. Rendl, M. Willnich, C. Alonso, A. Bergmann, and C. Reiners
Technical Evaluation of a New Immunoradiometric and a New Immunoluminometric Assay for Thyroglobulin
Clin. Chem., July 1, 2002; 48(7): 1077 - 1083.
[Abstract] [Full Text] [PDF]

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C. Calzolari, P.-Y. Marquet, B. Pau, C. A. Spencer, and M. Takeuchi
Thyroglobulin IRMA Pasteur Immunoassay: Sensitivity of the Assay and Interference from Thyroglobulin Autoantibodies • The authors of the above-mentioned article and editorial reply:
Clin. Chem., February 1, 1997; 43(2): 413 - 415.
[Full Text]


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