Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 42: 263-269, 1996;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mehta, H. B.
Right arrow Articles by Ullman, E. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mehta, H. B.
Right arrow Articles by Ullman, E. F.

Clinical Chemistry, Vol 42, 263-269, Copyright © 1996 by American Association for Clinical Chemistry

DELISA: sensitive nonisotopic assay for GAD65 autoantibodies, a key risk-assessment marker for insulin-dependent diabetes mellitus

HB Mehta, BS Vold, S Minkin and EF Ullman
Behring Diagnostics Inc., Syva Products Group, San Jose, CA 95161-9013, USA.

Nonisotopic assays for the measurement of autoantibodies to 65-kDa glutamic acid decarboxylase (GAD65) have not previously achieved performance equivalent to radiobinding assays (RBA). We have developed a modified ELISA protocol, DELISA, for measuring autoantibodies to GAD65 in serum. The method overcomes the problems of poor sensitivity and specificity associated with conventional ELISAs. Serum containing GAD65 autoantibodies is incubated with biotinylated GAD65 (bGAD65). Sufficient soluble Protein A-dextran conjugate is added to bind the immunoglobulins in the sample, including GAD65 autoantibodies to which GAD65 is bound. After incubation, the mixture is transferred to a streptavidin-p4ated microtiter well, which binds free bGAD65 but not bGAD65 bound to autoantibodies. Streptavidin-bound bGAD65 is detected by means of a peroxidase-GAS65MAb conjugate. The method appears to have comparable sensitivity and specificity to those of RBAs. Reaction of the antibodies with soluble antigen to increase the binding rate and the use of high serum concentrations and very low antigen concentrations to increase sensitivity are critical elements of the method.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
M. Ankelo, A. Westerlund-Karlsson, J. Ilonen, M. Knip, K. Savola, P. Kankaanpaa, L. Merio, H. Siitari, and A. Hinkkanen
Time-resolved Fluorometric Assay for Detection of Autoantibodies to Glutamic Acid Decarboxylase (GAD65)
Clin. Chem., June 1, 2003; 49(6): 908 - 915.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Westerlund-Karlsson, K. Suonpaa, M. Ankelo, J. Ilonen, M. Knip, and A. E. Hinkkanen
Detection of Autoantibodies to Protein Tyrosine Phosphatase-like Protein IA-2 with a Novel Time-resolved Fluorimetric Assay
Clin. Chem., June 1, 2003; 49(6): 916 - 923.
[Abstract] [Full Text] [PDF]

Home page
 Clin. DiabetesHome page
W. E. Winter, N. Harris, and D. Schatz
Immunological Markers in the Diagnosis and Prediction of Autoimmune Type 1a Diabetes
Clin. Diabetes, October 1, 2002; 20(4): 183 - 191.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Zeidler, L. J. Raffel, G. Costin, S. J. Shaw, T. A. Buchanan, J. Noble, J. I. Rotter, J. Palmer, J. P. Krischer, C. Wait, et al.
Autoantibodies and Human Leucocyte Antigen Class II in First-Degree Family Members of Mexican-American Type 1 Diabetic Patients
J. Clin. Endocrinol. Metab., October 1, 2001; 86(10): 4957 - 4962.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the American Association for Clinical Chemistry.