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Clinical Chemistry 42: 292-297, 1996;
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Clinical Chemistry, Vol 42, 292-297, Copyright © 1996 by American Association for Clinical Chemistry

Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography

HH Yao and WH Porter
Department of Pathology and Laboratory Medicine, University of Kentucky Medical Center, Lexington, 40536-0084, USA.

We developed a gas-chromatographic procedure for the simultaneous determination of ethylene glycol (EG) and its major toxic metabolite, glycolic acid (GA), suitable for clinical use in instances of EG intoxication. After serum protein precipitation with acetonitrile (containing internal standard), the supernate is treated with 2,2- dimethoxypropane (containing dimethylformamide) to remove water, and the volume is then reduced by evaporation to <100 microL of dimethylformamide (but not to dryness). After trimethylsilyl derivatization, the resulting derivatives are analyzed by capillary column gas chromatography. Only 100 microL of serum is required and the entire determination, including calibrators and controls, takes <2 h. The method gives a linear response to at least 10 g/L EG and 5 g/L GA and has a limit of detection <10 mg/L. Intraassay CV is < or = 2.8% for EG (100 and 1000 mg/L) and GA (100 and 500 mg/L); between-day CV is < or = 6.5%. The absolute recovery from serum was 91% for EG and 77-82% for GA (200 and 2000 mg/L each). Relative to calibrators prepared bovine serum albumin (70 g/L), the recovery was 99-104% for EG (100 - 5000 mg/L) and 95-105% for GA (50 - 2500 mg/L). No clinically important interference was detected for >60 exogenous or endogenous compounds and drugs.


The following articles in journals at HighWire Press have cited this article:


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W. H. Porter, M. Crellin, P. W. Rutter, and P. Oeltgen
Interference by Glycolic Acid in the Beckman Synchron Method for Lactate: A Useful Clue for Unsuspected Ethylene Glycol Intoxication
Clin. Chem., June 1, 2000; 46(6): 874 - 875.
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A. D. Fraser, B. A. Wolf, and L. Shaw
Importance of Glycolic Acid Analysis in Ethylene Glycol Poisoning
Clin. Chem., August 1, 1998; 44(8): 1769 - 1770.
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