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Clinical Chemistry 42: 309-312, 1996;
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Clinical Chemistry, Vol 42, 309-312, Copyright © 1996 by American Association for Clinical Chemistry

Quantification of fecal carbohydrates by near-infrared reflectance analysis

J Stein, B Purschian, S Zeuzem, B Lembcke and WF Caspary
Division of Gastroenterology, Department of Internal Medicine, Johann- Wolfgang-Goethe-University, Frankfurt, Germany.

Established methods for quantitative analysis of fecal carbohydrates (CHO) are time consuming, require unpopular sample handling, and are therefore rarely performed. The aim of this study was to evaluate the efficiency, validity, and practicability of near-infrared reflectance analysis (NIRA), compared with standard methods for measurement of fecal CHO. Excretion of fecal CHO was cross- validated spectrophotometrically with the anthrone method. Fecal CHO concentrations ranged form 2.7 to 24.5 g/kg wet weight. Methods comparison showed linear regression over the entire range of diagnostic relevance with a correlation coefficient of 0.869 (S(y/x) +/- 0.31). Repeated measurements from the same stool collections obviated the need for homogenization. These results indicate that NIRA may be a new, reliable, and accurate test in the diagnosis of CHO malabsorption.


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Water, fat, nitrogen, and sugar content in feces: reference intervals in children
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