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Clinical Chemistry, Vol 42, 738-743, Copyright © 1996 by American Association for Clinical Chemistry
N Harris, V Galpchian and N Rifai
Department of Laboratory Medicine, Children's Hospital and Harvard Medical School, Boston, MA 02115, USA.
We compared the performance of three methods for quantifying high- density lipoprotein cholesterol (HDL-C) with the Reference Method for HDL-C, using samples with a wide range of triglyceride (TG) concentrations (290-18000 mg/L). All three comparison assays-- utilizing a magnetic dextran sulfate precipitating reagent, a direct method, and a standard MgCl2-dextran sulfate reagent--were precise, with a run-to-run CV of less than or equal to 4.1%. However, the systematic error of these assays exceeded the National Cholesterol Education Program (NCEP) performance goal of less than or equal to 10% in half of the concentration ranges tested. Nevertheless, the total error of the assays generally meets the current 22% limit set by the NCEP. Although both the magnetic dextran sulfate precipitation reagent and the direct assay can be performed more rapidly than the MgCl2- dextran sulfate assay, the direct assay involves no sample preparation and requires only 4 microL of sample excluding the dead space. Although precipitation is frequently inadequate with the MgCl2-dextran sulfate reagent at TG concentrations >6000 mg/L, both the magnetic and the direct reagent show no interference from high TG concentrations as great as 18 000 mg/L.
The following articles in journals at HighWire Press have cited this article:
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  S. S. Levinson High Density- and Beta-Lipoprotein Screening for Risk of Coronary Artery Disease in the Context of New Findings on Reverse Cholesterol Transport Ann. Clin. Lab. Sci., April 1, 2002; 32(2): 123 - 136. [Abstract] [Full Text] [PDF]
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F. Gomez, J. Camps, J. M. Simo, N. Ferre, and J. Joven Agreement Study of Methods Based on the Elimination Principle for the Measurement of LDL- and HDL-Cholesterol Compared with Ultracentrifugation in Patients with Liver Cirrhosis Clin. Chem., August 1, 2000; 46(8): 1188 - 1191. [Full Text] [PDF]
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M.-L. Arranz-Pena, J. Tasende-Mata, and F. J. Martin-Gil Comparison of two homogeneous assays with a precipitation method and an ultracentrifugation method for the measurement of HDL-cholesterol Clin. Chem., December 1, 1998; 44(12): 2499 - 2505. [Abstract] [Full Text] [PDF]
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M. Nauck, W. Marz, and H. Wieland New immunoseparation-based homogeneous assay for HDL-cholesterol compared with three homogeneous and two heterogeneous methods for HDL-cholesterol Clin. Chem., July 1, 1998; 44(7): 1443 - 1451. [Abstract] [Full Text] [PDF]
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N. Rifai, T. G. Cole, E. Iannotti, T. Law, M. Macke, R. Miller, D. Dowd, and D. A. Wiebe Assessment of interlaboratory performance in external proficiency testing programs with a direct HDL-cholesterol assay Clin. Chem., July 1, 1998; 44(7): 1452 - 1458. [Abstract] [Full Text] [PDF]
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J. M a Simo, I. Castellano, N. Ferre, J. Joven, and J. Camps Evaluation of a homogeneous assay for high-density lipoprotein cholesterol: limitations in patients with cardiovascular, renal, and hepatic disorders Clin. Chem., June 1, 1998; 44(6): 1233 - 1241. [Abstract] [Full Text] [PDF]
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R. G. Reed In Search of the Ideal Measure of High-Density Lipoprotein Clin. Chem., October 1, 1997; 43(10): 1809 - 1810. [Full Text] [PDF]
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M. Nauck, W. Marz, J. Jarausch, C. Cobbaert, A. Sagers, D. Bernard, J. Delanghe, G. Honauer, P. Lehmann, E. Oestrich, et al. Multicenter evaluation of a homogeneous assay for HDL-cholesterol without sample pretreatment Clin. Chem., September 1, 1997; 43(9): 1622 - 1629. [Abstract] [Full Text] [PDF]
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Y.-C. Huang, J.-T. Kao, and K.-S. Tsai Evaluation of two homogeneous methods for measuring high-density lipoprotein cholesterol Clin. Chem., June 1, 1997; 43(6): 1048 - 1055. [Abstract] [Full Text] [PDF]
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N. Harris, V. Galpchian, J. Thomas, E. Iannotti, T. Law, and N. Rifai Three generations of high-density lipoprotein cholesterol assays compared with ultracentrifugation/dextran sulfate–Mg2+ method Clin. Chem., May 1, 1997; 43(5): 816 - 823. [Abstract] [Full Text] [PDF]
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P. N. M. Demacker, M. Hessels, H. Toenhake-Dijkstra, and H. Baadenhuijsen Precipitation methods for high-density lipoprotein cholesterol measurement compared, and final evaluation under routine operating conditions of a method with a low sample-to-reagent ratio Clin. Chem., April 1, 1997; 43(4): 663 - 668. [Abstract] [Full Text] [PDF]
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