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Clinical Chemistry 42: 744-748, 1996;
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Clinical Chemistry, Vol 42, 744-748, Copyright © 1996 by American Association for Clinical Chemistry

Determination of ionized magnesium in platelets and correlation with selected variables

JE Niemela, BM Snader and RJ Elin
Clinical Pathology Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.

We describe a method for determining the intracellular ionized magnesium concentration ([Mg2+]i) in platelets by using the fluorescent probe FURAPTRA. We determined the dissociation constant (KD) of FURAPTRA for Mg2+ (2.26 +/- 0.29 mmol/L), within-day assay variability (CV = 6.8%), among-day intraindividual variability (CV = 11.0%), variability after a 4-h delay in processing the blood specimen (t = 1.2, P >0.2; F = 6.2, P <0.02), and the reference interval (0.23-0.59 mmol/L) for this assay. We also evaluated the correlation between platelet [Mg2+]i and concentrations of selected serum electrolytes, proteins, and total cholesterol; age; body mass index; and gender. Only the inverse correlation between platelet [Mg2+]i and serum total cholesterol concentration in men was significant (r=-0.66, P <0.005).


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
H. J. Huijgen, R. Sanders, R. W. van Olden, M. G. Klous, F. R. Gaffar, and G. T. B. Sanders
Intracellular and extracellular blood magnesium fractions in hemodialysis patients; is the ionized fraction a measure of magnesium excess?
Clin. Chem., March 1, 1998; 44(3): 639 - 648.
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