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Clinical Chemistry 42: 910-914, 1996;
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Clinical Chemistry, Vol 42, 910-914, Copyright © 1996 by American Association for Clinical Chemistry

Methylmalonic acid quantification by stable isotope dilution gas chromatography-mass spectrometry from filter paper urine samples

MT McCann, MM Thompson, IC Gueron, B Lemieux, R Giguere and M Tuchman
Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.

A specific method for quantification of methylmalonic acid (MMA) from urine samples dried onto filter paper is described. The method involves stable isotope dilution gas chromatography-mass spectrometry with [methyl-2H3]-MMA as the internal standard. MMA is stable in dry paper samples stored at room temperature for at least 2 weeks. The extraction efficiency of MMA from paper was 56-58%. The concentration of urinary MMA in dried filter paper specimens from 190 normal controls was 1.21 +/- 1.34 (mean +/- SD) mmol/mol of urinary creatinine. Age-related reference values are also reported. The concentrations, normalized to the urinary creatinine concentration, decrease with age. The applicability of this method to rapid screening for cobalamin (vitamin B12)-related disorders and methylmalonic aciduria is demonstrated.


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A. Schmedes and I. Brandslund
Analysis of methylmalonic Acid in plasma by liquid chromatography-tandem mass spectrometry.
Clin. Chem., April 1, 2006; 52(4): 754 - 757.
[Abstract] [Full Text] [PDF]

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M. M. Kushnir, G. Komaromy-Hiller, B. Shushan, F. M. Urry, and W. L. Roberts
Analysis of Dicarboxylic Acids by Tandem Mass Spectrometry. High-Throughput Quantitative Measurement of Methylmalonic Acid in Serum, Plasma, and Urine
Clin. Chem., November 1, 2001; 47(11): 1993 - 2002.
[Abstract] [Full Text] [PDF]


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Copyright © 1996 by the American Association for Clinical Chemistry.