| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Clinical Chemistry, Vol 42, 955-964, Copyright © 1996 by American Association for Clinical Chemistry
AA Franke and LJ Custer
Cancer Research Center of Hawaii, Honolulu, HI 96813, USA. adrian@crch.hawaii.edu
Soy isoflavones were quantified from human milk by a fast, precise, and selective HPLC method after enzymatic hydrolysis of conjugated isoflavones and extraction with ethyl acetate. Isoflavone aglycones and their mammalian metabolites equol and O-desmethylangolensin were separated selectively and identified by absorbance patterns, fluorometric and electrochemical detection, gas chromatography-mass spectrometric analysis after trimethylsilylation, and with internal and external authentic standards. HPLC injections of 20 microL of human milk showed detection limits of 1-3 pmol for all analytes by using diode-array detection. The detection limit could be improved by as much as 1000-fold by extended concentration through partitioning with ethyl acetate, by using electrochemical detection, by increasing the injection volumes, or by combining these techniques. We used the proposed method to monitor isoflavone concentrations in human milk and in human urine after challenge with 5, 10, and 20 g of roasted soybeans in the diet. Implications of the results for the potential of isoflavones to prevent cancer in newborn infants exposed to these agents are discussed.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  J. Mardon, J. Mathey, S. Kati-Coulibaly, C. Puel, M.-J. Davicco, P. Lebecque, M.-N. Horcajada, and V. Coxam Influence of Lifelong Soy Isoflavones Consumption on Bone Mass in the Rat Experimental Biology and Medicine, February 1, 2008; 233(2): 229 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A Franke, B. M Halm, L. J Custer, Y. Tatsumura, and S. Hebshi Isoflavones in breastfed infants after mothers consume soy. Am. J. Clinical Nutrition, August 1, 2006; 84(2): 406 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Guo, R. P. Chi, D. R. Germolec, and K. L. White Jr. Stimulation of the Immune Response in B6C3F1 Mice by Genistein Is Affected by Exposure Duration, Gender, and Litter Order J. Nutr., October 1, 2005; 135(10): 2449 - 2456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Merritt and B. H. Jenks Safety of Soy-Based Infant Formulas Containing Isoflavones: The Clinical Evidence J. Nutr., May 1, 2004; 134(5): 1220S - 1224S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zubik and M. Meydani Bioavailability of soybean isoflavones from aglycone and glucoside forms in American women Am. J. Clinical Nutrition, June 1, 2003; 77(6): 1459 - 1465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lampe Isoflavonoid and Lignan Phytoestrogens as Dietary Biomarkers J. Nutr., March 1, 2003; 133(3): 956S - 964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Badger, M. J. J. Ronis, R. Hakkak, J. C. Rowlands, and S. Korourian The Health Consequences of Early Soy Consumption J. Nutr., March 1, 2002; 132(3): 559S - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. B. Patisaul, M. Dindo, P. L. Whitten, and L. J. Young Soy Isoflavone Supplements Antagonize Reproductive Behavior and Estrogen Receptor {{alpha}}- and {beta}-Dependent Gene Expression in the Brain Endocrinology, July 1, 2001; 142(7): 2946 - 2952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Izumi, M. K. Piskula, S. Osawa, A. Obata, K. Tobe, M. Saito, S. Kataoka, Y. Kubota, and M. Kikuchi Soy Isoflavone Aglycones Are Absorbed Faster and in Higher Amounts than Their Glucosides in Humans J. Nutr., July 1, 2000; 130(7): 1695 - 1699. [Abstract] [Full Text]
|
|
|
|
|
|
S. Ji, G. M. Willis, G. R. Frank, S. G. Cornelius, and M. E. Spurlock Soybean Isoflavones, Genistein and Genistin, Inhibit Rat Myoblast Proliferation, Fusion and Myotube Protein Synthesis J. Nutr., July 1, 1999; 129(7): 1291 - 1297. [Abstract] [Full Text]
|
|
|
|
|
|
K. D. R. Setchell and A. Cassidy Dietary Isoflavones: Biological Effects and Relevance to Human Health J. Nutr., March 1, 1999; 129(3): 758 - 758. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. M. Tham, C. D. Gardner, and W. L. Haskell Potential Health Benefits of Dietary Phytoestrogens: A Review of the Clinical, Epidemiological, and Mechanistic Evidence J. Clin. Endocrinol. Metab., July 1, 1998; 83(7): 2223 - 2235. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. M. Sheehan, A. Franke, L. J. C. Bluck, S. A. Bingham, and J. Slavin Isoflavone Content of Breast Milk and Soy Formulas: Benefits and Risks • The author of the Editorial referred to replies to the above Letters: Clin. Chem., May 1, 1997; 43(5): 850 - 852. [Full Text]
|
|
|
|
|
|
J. Slavin More Information on Phytoestrogens in Breast Milk Clin. Chem., March 1, 1997; 43(3): 548 - 549. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
