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Clinical Chemistry 42: 1159-1167, 1996;
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Clinical Chemistry, Vol 42, 1159-1167, Copyright © 1996 by American Association for Clinical Chemistry

Development of a two-site solid-phase immunochemiluminescent assay for measurement of dimeric inhibin-A in human serum and other biological fluids

DS McConnell, V Padmanabhan, TB Pollak, NP Groome, JJ Ireland and AR Midgley Jr
Reproductive Sciences Program, University of Michigan, Ann Arbor 48109- 0404, USA. DanMcC@umich.edu

Inhibin is a heterodimeric glycoprotein that inhibits the secretion of follitropin from the pituitary and has been isolated in two distinct forms composed of a common alpha subunit and either a beta A or beta B subunit. Utilizing paired monoclonal antibodies specific to the alpha and beta A subunit, we have developed an immunochemiluminescent assay for dimeric inhibin-A. The assay is capable of quantifying free and bound inhibin-A in human serum and follicular fluid. The limit of detection is 10 ng/L. Related proteins exhibit little cross-reactivity or interference. Recovery is excellent. Whereas samples from men and postmenopausal women are near the detection limit of the assay, inhibin- A is higher in the luteal than the follicular phase of normally cycling women, 20-fold higher during in vitro fertilization treatment, and approximately 200-fold greater in pregnancy. The assay measures inhibin- A in follicular fluid from a variety of other species.


The following articles in journals at HighWire Press have cited this article:


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N. E. Reame, T. L. Wyman, D. J. Phillips, D. M. de Kretser, and V. Padmanabhan
Net Increase in Stimulatory Input Resulting from a Decrease in Inhibin B and an Increase in Activin A May Contribute in Part to the Rise in Follicular Phase Follicle-Stimulating Hormone of Aging Cycling Women
J. Clin. Endocrinol. Metab., September 1, 1998; 83(9): 3302 - 3307.
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W. Byrd, M. J. Bennett, B. R. Carr, Y. Dong, F. Wians, and W. Rainey
Regulation of Biologically Active Dimeric Inhibin A and B From Infancy to Adulthood in the Male
J. Clin. Endocrinol. Metab., August 1, 1998; 83(8): 2849 - 2854.
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T. M. Plant, V. Padmanabhan, S. Ramaswamy, D. S. McConnell, S. J. Winters, N. Groome, A. R. Midgley Jr., and A. S. McNeilly
Circulating Concentrations of Dimeric Inhibin A and B in the Male Rhesus Monkey (Macaca mulatta)
J. Clin. Endocrinol. Metab., August 1, 1997; 82(8): 2617 - 2621.
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P. Pigny, R. Desailloud, C. Cortet-Rudelli, A. Duhamel, D. Deroubaix-Allard, A. Racadot, and D. Dewailly
Serum {alpha}-Inhibin Levels in Polycystic Ovary Syndrome: Relationship to the Serum Androstenedione Level
J. Clin. Endocrinol. Metab., June 1, 1997; 82(6): 1939 - 1943.
[Abstract] [Full Text] [PDF]


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