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Clinical Chemistry, Vol 42, 1182-1188, Copyright © 1996 by American Association for Clinical Chemistry
N Harris, EJ Neufeld, JW Newburger, B Ticho, A Baker, GS Ginsburg, E Rimm and N Rifai
Department of Laboratory Medicine, Children's Hospital, Boston, MA 02115, USA.
This study compares a new latex immunoseparation method for the direct determination of plasma low-density lipoprotein cholesterol (LDL-C) with the reference procedure for LDL-C (beta-quantification) in a pediatric hyperlipidemic population. The direct LDL-C assay has a mean bias of -98 mg/L in a fasting group (n = 96) of patients (mean triglycerides 1057 +/- 720 mg/L) and a bias of +177 mg/L in a nonfasting group (n = 42, mean triglycerides 4854 +/- 5457 mg/L). The mean total analytical error calculated from our data is 13.8%. The direct LDL-C assay and the commonly used Friedewald calculation respectively classified 81% and 84% of fasting patients correctly, according to the cutoffs of 1100 and 1300 mg/L for LDL-C set by the National Cholesterol Education Program for pediatric patients. Of combined fasting and nonfasting patients, 80% were correctly classified by the direct LDL-C assay. Therefore, despite several analytical shortcomings, the direct LDL-C assay may be useful in managing hyperlipidemic children without the need for a fasting specimen.
The following articles in journals at HighWire Press have cited this article:
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  E. M. Haney, L. H. Huffman, C. Bougatsos, M. Freeman, R. D. Steiner, and H. D. Nelson Screening and Treatment for Lipid Disorders in Children and Adolescents: Systematic Evidence Review for the US Preventive Services Task Force Pediatrics, July 1, 2007; 120(1): e189 - e214. [Abstract] [Full Text] [PDF]
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M. Esteban-Salan, A. Guimon-Bardesi, J. M. de la Viuda-Unzueta, M. N. Azcarate-Ania, P. Pascual-Usandizaga, and E. Amoroto-Del-Rio Analytical and Clinical Evaluation of Two Homogeneous Assays for LDL-Cholesterol in Hyperlipidemic Patients Clin. Chem., August 1, 2000; 46(8): 1121 - 1131. [Abstract] [Full Text] [PDF]
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F. Gomez, J. Camps, J. M. Simo, N. Ferre, and J. Joven Agreement Study of Methods Based on the Elimination Principle for the Measurement of LDL- and HDL-Cholesterol Compared with Ultracentrifugation in Patients with Liver Cirrhosis Clin. Chem., August 1, 2000; 46(8): 1188 - 1191. [Full Text] [PDF]
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 B. S. Ticho, E. J. Neufeld, J. W. Newburger, N. Harris, A. Baker, and N. Rifai Utility of Direct Measurement of Low-Density Lipoprotein Cholesterol in Dyslipidemic Pediatric Patients Arch Pediatr Adolesc Med, August 1, 1998; 152(8): 787 - 791. [Abstract] [Full Text] [PDF]
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N. Rifai, T. G. Cole, E. Iannotti, T. Law, M. Macke, R. Miller, D. Dowd, and D. A. Wiebe Assessment of interlaboratory performance in external proficiency testing programs with a direct HDL-cholesterol assay Clin. Chem., July 1, 1998; 44(7): 1452 - 1458. [Abstract] [Full Text] [PDF]
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N. Rifai, E. Iannotti, K. DeAngelis, and T. Law Analytical and clinical performance of a homogeneous enzymatic LDL-cholesterol assay compared with the ultracentrifugation-dextran sulfate-Mg2+ method Clin. Chem., June 1, 1998; 44(6): 1242 - 1250. [Abstract] [Full Text] [PDF]
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