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Clinical Chemistry, Vol 42, 1196-1201, Copyright © 1996 by American Association for Clinical Chemistry
T Lovgren, L Merio, K Mitrunen, ML Makinen, M Makela, K Blomberg, T Palenius and K Pettersson
Department of Biotechnology, University of Turku, Finland. timo.lovgren@utu
The availability of an intrinsically fluorescent, inert, and stable Eu chelate label made it feasible to design one-step all-in-one immunoassays with time-resolved fluorometry for detection. Both competitive and noncompetitive immunoassays are performed in microtitration wells containing all assay-specific components in a stable dry form. Only the sample and one assay buffer common for all analytes need to be added. Model assays for human chorionic gonadotropin (hCG), alpha-fetoprotein (AFP), and progesterone all reached equilibrium in 15 min or less without compromising the performance characteristics of the measurements, all of which perform at least equivalent to state-of-the-art assays. The detection limits for hCG, AFP, and progesterone were 0.3 IU/L, 0.1 microgram/L, and 0.5 nmol/L, respectively. The assay ranges for hCG and AFP were linear to 5000 IU/L and 1200 micrograms/L, respectively. The immunoassay format can be readily implemented in a fully automated random-access immunoassay system with optimal performance characteristics and no handling of analyte-specific assay components.
The following articles in journals at HighWire Press have cited this article:
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  S. Eriksson, T. Ilva, C. Becker, J. Lund, P. Porela, K. Pulkki, L.-M. Voipio-Pulkki, and K. Pettersson Comparison of Cardiac Troponin I Immunoassays Variably Affected by Circulating Autoantibodies Clin. Chem., May 1, 2005; 51(5): 848 - 855. [Abstract] [Full Text] [PDF]
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P. Huhtinen, A.-M. Pelkkikangas, S. Jaakohuhta, T. Lovgren, and H. Harma Quantitative, Rapid Europium(III) Nanoparticle-Label-Based All-in-One Dry-Reagent Immunoassay for Thyroid-Stimulating Hormone Clin. Chem., October 1, 2004; 50(10): 1935 - 1936. [Full Text] [PDF]
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F. Pagani, F. Stefini, and M. Panteghini Innotrac Aio! Second-Generation Cardiac Troponin I Assay: Imprecision Profile and Other Key Characteristics for Clinical Use Clin. Chem., July 1, 2004; 50(7): 1271 - 1272. [Full Text] [PDF]
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P. von Lode, J. Rainaho, and K. Pettersson Quantitative, Wide-Range, 5-Minute Point-of-Care Immunoassay for Total Human Chorionic Gonadotropin in Whole Blood Clin. Chem., June 1, 2004; 50(6): 1026 - 1035. [Abstract] [Full Text] [PDF]
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 A. H. Peruski and L. F. Peruski Jr. Immunological Methods for Detection and Identification of Infectious Disease and Biological Warfare Agents Clin. Vaccine Immunol., July 1, 2003; 10(4): 506 - 513. [Full Text] [PDF]
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Q.-P. Qin, O. Peltola, and K. Pettersson Time-resolved Fluorescence Resonance Energy Transfer Assay for Point-of-Care Testing of Urinary Albumin Clin. Chem., July 1, 2003; 49(7): 1105 - 1113. [Abstract] [Full Text] [PDF]
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Q.-P. Qin, M. Christiansen, and K. Pettersson Point-of-Care Time-resolved Immunofluorometric Assay for Human Pregnancy-associated Plasma Protein A: Use in First-Trimester Screening for Down Syndrome Clin. Chem., March 1, 2002; 48(3): 473 - 483. [Abstract] [Full Text] [PDF]
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T. Soukka, J. Paukkunen, H. Harma, S. Lonnberg, H. Lindroos, and T. Lovgren Supersensitive Time-resolved Immunofluorometric Assay of Free Prostate-specific Antigen with Nanoparticle Label Technology Clin. Chem., July 1, 2001; 47(7): 1269 - 1278. [Abstract] [Full Text] [PDF]
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