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Clinical Chemistry 42: 1206-1209, 1996;
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Clinical Chemistry, Vol 42, 1206-1209, Copyright © 1996 by American Association for Clinical Chemistry

Rapid method for visual identification of specific DNA sequences based on DNA-tagged liposomes

GS Rule, RA Montagna and RA Durst
Analytical Chemistry Laboratories, Cornell University, Geneva, NY 14456- 0462, USA.

We describe a rapid method for visually determining specific DNA sequences at femtomole concentrations. Liposomes, encapsulating a red dye and labeled with oligonucleotide, were used in a capillary migration-sandwich hybridization assay. Capture probe was immobilized on nitrocellulose strips, and liposomes, migrating along each strip, formed a visually discernible band in the presence of target DNA. One femtomole of synthetic target sequence could be detected in < 10 min. Sufficiently stringent hybridization conditions can be used to allow the discrimination of a 10% mismatch sequence from perfectly complementary DNA. A 366-base PCR product was detected at 200 fmol.


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