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Clinical Chemistry 42: 1450-1453, 1996;
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Clinical Chemistry, Vol 42, 1450-1453, Copyright © 1996 by American Association for Clinical Chemistry

Interference of soluble TNF-alpha receptors in immunological detection of tumor necrosis factor-alpha

E Moreau, J Philippe, S Couvent and G Leroux-Roels
Department of Clinical Chemistry, Microbiology, and Immunology, University Hospital Gent, Belgium.

We have studied the interference of soluble tumor necrosis factor-alpha receptor p55 (sTNF-R55) on the quantification of tumor necrosis factor- alpha (TNF-alpha) in three immunoassays: the commercially available enzyme amplified-sensitivity immunoassay (EASIA) of Medgenix and the ELISA of Boehringer Mannheim, and an assay based on electrochemiluminescence (ECL) technology. TNF-alpha measurements by EASIA and ELISA were not affected by the presence of sTNF-R55, but results by the ECL method were clearly influenced by the receptor. We then performed a second set of experiments with the ECL system to examine what factors might influence the importance of interference from sTNF-R55 and soluble TNF-alpha receptor p75 (sTNF-R75), which influences TNF-alpha measurements the same as sTNF-R55. We found that extending the incubation time and increasing the concentration of capture antibody decreased the interfering capacity of sTNF-R55 and sTNF-R75. A clear insight into these phenomena is of utmost importance for correct interpretation of cytokine quantification.


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