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Clinical Chemistry 42: 1547-1555, 1996;
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Clinical Chemistry, Vol 42, 1547-1555, Copyright © 1996 by American Association for Clinical Chemistry

Detection of immobilized amplicons by ELISA-like techniques

AA Oroskar, SE Rasmussen, HN Rasmussen, SR Rasmussen, BM Sullivan and A Johansson
Nalge Nunc International, Naperville, IL 60563-1796, USA.

The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'- phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound oligonucleotides are also utilized as capture probe in the hybridization-based signal amplification for detection of an infectious organism.


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