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Evaluation of urinary metanephrine and normetanephrine enzyme immunoassay (ELISA) kits by comparison with isotope dilution mass spectrometry

¹ Central Laboratory for Clinical Chemistry, University Hospital, P.O. Box 30.001, 9700 RB Groningen, The Netherlands.

² Repromed GmbH, Flughafenstraße 52A, D-22335 Hamburg, Germany.
^a Author for correspondence. Fax 31-50-3612290.

Determination of urinary 3-O-methylated catecholamines (metanephrines) is generally considered a principal test for the clinical chemical diagnosis of pheochromocytoma and is currently performed predominantly with chromatographic techniques such as gas–liquid chromatography and HPLC. Enzyme immunoassays based on microtiter plate technology have recently been developed for the quantitative determination of urinary metanephrine (M) and normetanephrine (NM). We compared the results for urinary M and NM determined by these ELISA methods with those obtained by a recently developed isotope dilution mass spectrometric method. From this comparative study we can conclude that the investigated ELISA methods are applicable in the quantification of urinary M and thus can be successfully used to establish the diagnosis of pheochromocytoma. These relatively simple methods can be executed in any clinical laboratory and in time may replace the present, more complicated, chromatographic techniques.

Key Words: indexing terms: gas chromatography • mass spectrometry • pheochromocytoma

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