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Clinical Chemistry 43: 146-153, 1997;
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(Clinical Chemistry. 1997;43:146-153.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Reference values for plasma concentrations of vitamin E and A and carotenoids in a Swiss population from infancy to adulthood, adjusted for seasonal influences

Brigitte M. Winklhofer-Roob1,a, Martin A. van't Hof2 and David H. Shmerling1

1 Division of Gastroenterology and Nutrition, Department of Pediatrics, University of Zurich, Switzerland.

2 Medical Statistics Department, University of Nijmegen, The Netherlands.
a Address for correspondence: Institute of Biochemistry, Karl-Franzens University of Graz, Schubertstr. 1, A-8010 Graz, Austria. Fax 43-316-380-9845.

In a cross-sectional survey, plasma concentrations of {alpha}- and {gamma}-tocopherol, {alpha}- and ß-carotene (cis and trans isomers), lycopene, and retinol were determined by reversed-phase HPLC, and ratios of plasma {alpha}-tocopherol to cholesterol were calculated in 208 Swiss individuals ages 0.4–38.7 years. The influence of age, sex, and season of sampling was studied. Age was a significant predictor of all plasma concentrations except {alpha}-carotene. No sex-related differences were observed. Season of sampling affected {alpha}-tocopherol and retinol (higher in winter) and {gamma}-tocopherol and cholesterol concentrations (higher in winter and spring than in the other seasons). After correction for seasonal influences, age differences were 0.24 µmol/L per year for {alpha}-tocopherol, 0.04 µmol/L per year for retinol, and 0.04 µmol/L per year for cholesterol concentrations; ratios of plasma {alpha}-tocopherol to cholesterol were not affected by age. We constructed age-specific reference intervals from the regression line and a multiple of the standard deviation. Separate regression equations are presented for seasons with low and high values.


Key Words: indexing terms: tocopherol • lycopene • retinol • carotene • cholesterol • age-related effects • pediatric chemistry • variation, source of • epidemiology




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