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Clinical Chemistry 43: 154-157, 1997;
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(Clinical Chemistry. 1997;43:154-157.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Ascorbic acid determination with an automated enzymatic procedure

Winnie Lee, Susan M. Roberts and Robert F. Labbea

Department of Laboratory Medicine, Box 359743, Harborview Medical Center and University of Washington, Seattle, WA 98104-2499.
a Author for correspondence. Fax 206-731-3930; e-mail labbe{at}mail.labmed washington.edu.

Given the widespread interest in antioxidant nutrients, we have developed a new procedure that will permit the automated determination of plasma ascorbic acid (AA) concentration with a Roche Fara centrifugal analyzer. After the deproteinization of plasma with metaphosphoric acid, AA is oxidized to dehydroascorbic acid by AA oxidase. The product is coupled to o-phenylenediamine to produce a chromophore for which the absorbance is measured at 340 nm. The procedure allows much faster throughput than conventional HPLC methods while yielding results that correlate well and provide improved precision. Linearity extends beyond the reference range of 26.1–84.6 µmol/L, and severe hemolysis is the only interference identified.


Key Words: indexing terms: antioxidants • centrifugal analyzer • dehydroascorbic acid




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Copyright © 1997 by the American Association for Clinical Chemistry.