Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 40-44, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (7)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Reyes, A. A.
Right arrow Articles by Wallace, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Reyes, A. A.
Right arrow Articles by Wallace, R. B.
Related Collections
Right arrow Molecular Diagnostics and Genetics
Right arrow Hematology
(Clinical Chemistry. 1997;43:40-44.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Ligase chain reaction assay for human mutations: the Sickle Cell by LCR assay

Antonio A. Reyes1, Paola Carrera2, Elena Cardillo2, Luis Ugozzoli1, Jimmie D. Lowery1, Ching-I P. Lin1, Matthew Go3, Maurizio Ferrari2 and R. Bruce Wallace1,a

1 DNA Diagnostics Business Unit, and
2 IRCCS Clinical Molecular Biology Laboratory, H. San Raffaele, via Olgettina 60, 20132 Milan, Italy.

3 Clinical Diagnostics Group, Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547.
a Author for correspondence. Fax 510-741-5811; e-mail bwallace{at}bio-rad.com

We can detect the ß-globin gene sickle cell mutation by using an assay based on the ligase chain reaction. The simultaneous amplification of the human growth hormone gene in the same reaction serves as a control for the amount of template DNA or amplification efficiency. Ligation products, which are biotinylated at one end and tagged with an arbitrary "tail" sequence at the other, are captured by hybridization to "tail"-complementary oligonucleotides immobilized on polystyrene microwells. The captured ligation products are detected colorimetrically by use of streptavidin–alkaline phosphatase conjugate. In a study of 24 subjects, the assay unequivocally discriminated among normal, carrier, and sickle cell genotypes.


Key Words: indexing terms: hemoglobinopathies • DNA amplification • genetic diseases • human growth hormone gene • biotin–streptavidin interaction




The following articles in journals at HighWire Press have cited this article:


Home page
 Nucleic Acids ResHome page
A. K. Tong and J. Ju
Single nucleotide polymorphism detection by combinatorial fluorescence energy transfer tags and biotinylated dideoxynucleotides
Nucleic Acids Res., March 1, 2002; 30(5): e19 - e19.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.