Clinical Chemistry
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Clinical Chemistry 43: 59-63, 1997;
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(Clinical Chemistry. 1997;43:59-63.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Development of ELISA to estimate thymosin {alpha}1, the N terminus of prothymosin {alpha}, in human tumors

Lourdes Loidi, Anxo Vidal, Juan B. Zalvide, José L. Puente1, Francisco Reyes1 and Fernando Domíngueza

1 Departamento de Cirugía, Facultad de Medicina, Hospital Xeral de Galicia, Santiago de Compostela, Spain.
a Author for correspondence. Fax (34) (81) 574145; e-mail fsfedopu{at}usc.es

We reported that tumor content of prothymosin {alpha} (ProT {alpha}) is a proliferation index of human breast tumors that might be used to identify patients at high risk for distant metastasis (Dominguez et al., Eur J Cancer 1993;29A:893–7). In that study ProT {alpha} concentrations were measured by a RIA; here we present an alternative nonisotopic assay that could be used in a standard clinical laboratory. Main features of the ELISA are: (a) A recombinant fusion protein glutathione S-transferase (GST)–human ProT {alpha} was used to coat the microtiter plates; (b) we used a polyclonal antiserum raised in rabbits that detects thymosin {alpha}1, the NH2-terminal fragment of ProT {alpha}; (c) it is as sensitive as the RIA; (d) it is faster than the RIA. ProT {alpha} concentrations in various human tumors (skin, esophagus, colorectal, and breast) as assessed by ELISA were comparable with, although twofold greater than, the values previously estimated by RIA.


Key Words: indexing terms: cancer • immunoassay • cell proliferation




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Immunocytological and Preliminary Immunohistochemical Studies of Prothymosin {alpha}, a Human Cancer-associated Polypeptide, With a Well-characterized Polyclonal Antibody
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