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Clinical Chemistry 43: 1814-1823, 1997;
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Right arrow Molecular Diagnostics and Genetics
(Clinical Chemistry. 1997;43:1814-1823.)
© 1997 American Association for Clinical Chemistry, Inc.

Review

Molecular diagnosis of B- and T-cell lymphomas: fundamental principles and clinical applications

William N. Rezuke, Evelyn C. Abernathy and Gregory J. Tsongalisa

Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT 06102.
a Author for correspondence. Fax 860-545-5206;

Molecular diagnostic assays have become routine in the evaluation of lymphoid malignancies. Both Southern transfer and polymerase chain reaction (PCR) technologies are used to assess for B- and T-cell clonality, the presence of rearrangements involving protooncogenes such as bcl-1 and bcl-2, and the monitoring of minimal residual disease. We review the fundamentals of B- and T-cell ontogeny as well as the basic principles of the Southern transfer and PCR assays and their applications to the diagnosis of lymphoid malignancies.




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Diagnosis of Canine Lymphoid Neoplasia Using Clonal Rearrangements of Antigen Receptor Genes
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N. J. Munro, K. Snow, J. A. Kant, and J. P. Landers
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Copyright © 1997 by the American Association for Clinical Chemistry.