| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
Department of Pathology and Laboratory of Molecular Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9072.
a Author for correspondence. Fax 214-648-4070; e-mail scheuerm{at}utsw.swmed.edu
Human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe or life-threatening illnesses such as shingles or lymphoproliferative disorders. We report the development and validation of a quantitative PCR method to measure viral burden for all eight human herpesviruses (HSV1, HSV2, VZV, EBV, CMV, HHV6, HHV7, and KSHV) in patients' samples. The method uses an internal standard that is coamplified with the viral target, allowing quantification of viral genomes in absolute terms (e.g., viral targets/mL of blood) and ruling out false-negative results. We demonstrate that transplant patients with lymphoproliferative disorder carry an EBV viral burden 3 logs higher than nontransplant patients. EBV titers in transplant patients without a lymphoproliferative disorder are between these values. This quantitative PCR method may aid in differentiating clinically significant vs latent viral burden in immunosuppressed patients.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  H. Hakim, C. Gibson, J. Pan, K. Srivastava, Z. Gu, M. J. Bankowski, and R. T. Hayden Comparison of Various Blood Compartments and Reporting Units for the Detection and Quantification of Epstein-Barr Virus in Peripheral Blood J. Clin. Microbiol., July 1, 2007; 45(7): 2151 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Habbal, F. Monem, and B. C. Gartner Errors in Published Sequences of Human Cytomegalovirus Primers and Probes: Do We Need More Quality Control? J. Clin. Microbiol., October 1, 2005; 43(10): 5408 - 5409. [Full Text] [PDF]
|
|
|
|
|
|
S. J. C. Stevens, S. A. W. M. Verkuijlen, B. Hariwiyanto, Harijadi, J. Fachiroh, D. K. Paramita, I. B. Tan, S. M. Haryana, and J. M. Middeldorp Diagnostic Value of Measuring Epstein-Barr Virus (EBV) DNA Load and Carcinoma-Specific Viral mRNA in Relation to Anti-EBV Immunoglobulin A (IgA) and IgG Antibody Levels in Blood of Nasopharyngeal Carcinoma Patients from Indonesia J. Clin. Microbiol., July 1, 2005; 43(7): 3066 - 3073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Burggraf and B. Olgemoller Simple Technique for Internal Control of Real-Time Amplification Assays Clin. Chem., May 1, 2004; 50(5): 819 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. L. Pang, L. Chui, J. Fenton, B. LeBlanc, and J. K. Preiksaitis Comparison of LightCycler-Based PCR, COBAS Amplicor CMV Monitor, and pp65 Antigenemia Assays for Quantitative Measurement of Cytomegalovirus Viral Load in Peripheral Blood Specimens from Patients after Solid Organ Transplantation J. Clin. Microbiol., July 1, 2003; 41(7): 3167 - 3174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jebbink, X. Bai, B. B. Rogers, D. B. Dawson, R. H. Scheuermann, and R. Domiati-Saad Development of Real-Time PCR Assays for the Quantitative Detection of Epstein-Barr Virus and Cytomegalovirus, Comparison of TaqMan Probes, and Molecular Beacons J. Mol. Diagn., February 1, 2003; 5(1): 15 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, J. S. Dummer, W. R. Estes, S. Meng, P. F. Wright, and Y.-W. Tang Measurement of Human Cytomegalovirus Loads by Quantitative Real-Time PCR for Monitoring Clinical Intervention in Transplant Recipients J. Clin. Microbiol., January 1, 2003; 41(1): 187 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. C. Stevens, S. A. W. M. Verkuijlen, A. J. C. v. d. Brule, and J. M. Middeldorp Comparison of Quantitative Competitive PCR with LightCycler-Based PCR for Measuring Epstein-Barr Virus DNA Load in Clinical Specimens J. Clin. Microbiol., November 1, 2002; 40(11): 3986 - 3992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. C. Stevens, I. Pronk, and J. M. Middeldorp Toward Standardization of Epstein-Barr Virus DNA Load Monitoring: Unfractionated Whole Blood as Preferred Clinical Specimen J. Clin. Microbiol., April 1, 2001; 39(4): 1211 - 1216. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. J. C. Stevens, E. A. M. Verschuuren, I. Pronk, W. van der Bij, M. C. Harmsen, T. H. The, C. J. L. M. Meijer, A. J. C. van den Brule, and J. M. Middeldorp Frequent monitoring of Epstein-Barr virus DNA load in unfractionated whole blood is essential for early detection of posttransplant lymphoproliferative disease in high-risk patients Blood, March 1, 2001; 97(5): 1165 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Jabs, H. Hennig, M. Kittel, K. Pethig, F. Smets, P. Bucsky, H. Kirchner, and H. J. Wagner Normalized Quantification by Real-Time PCR of Epstein-Barr Virus Load in Patients at Risk for Posttransplant Lymphoproliferative Disorders J. Clin. Microbiol., February 1, 2001; 39(2): 564 - 569. [Abstract] [Full Text]
|
|
|
|
|
|
M. L. Gulley Molecular Diagnosis of Epstein-Barr Virus-Related Diseases J. Mol. Diagn., February 1, 2001; 3(1): 1 - 10. [Abstract] [Full Text]
|
|
|
|
|
|
L. Schaade, P. Kockelkorn, K. Ritter, and M. Kleines Detection of Cytomegalovirus DNA in Human Specimens by LightCycler PCR J. Clin. Microbiol., November 1, 2000; 38(11): 4006 - 4009. [Abstract] [Full Text]
|
|
|
|
|
|
X. Bai, B. B. Rogers, P. C. Harkins, J. Sommerauer, R. Squires, K. Rotondo, A. Quan, D. B. Dawson, and R. H. Scheuermann Predictive Value of Quantitative PCR-Based Viral Burden Analysis for Eight Human Herpesviruses in Pediatric Solid Organ Transplant Patients J. Mol. Diagn., November 1, 2000; 2(4): 191 - 201. [Abstract] [Full Text]
|
|
|
|
|
|
S. J. C. Stevens, M. B. H. J. Vervoort, A. J. C. van den Brule, P. L. Meenhorst, C. J. L. M. Meijer, and J. M. Middeldorp Monitoring of Epstein-Barr Virus DNA Load in Peripheral Blood by Quantitative Competitive PCR J. Clin. Microbiol., September 1, 1999; 37(9): 2852 - 2857. [Abstract] [Full Text]
|
|
|
|
|
|
A. P. Limaye, M.-L. Huang, E. E. Atienza, J. M. Ferrenberg, and L. Corey Detection of Epstein-Barr Virus DNA in Sera from Transplant Recipients with Lymphoproliferative Disorders J. Clin. Microbiol., April 1, 1999; 37(4): 1113 - 1116. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. A. Hosler, R. O. Bash, X. Bai, V. Jain, and R. H. Scheuermann Development and Validation of a Quantitative Polymerase Chain Reaction Assay to Evaluate Minimal Residual Disease for T-Cell Acute Lymphoblastic Leukemia and Follicular Lymphoma Am. J. Pathol., April 1, 1999; 154(4): 1023 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Kuker, L. Schaade, K. Ritter, and W. Nacimiento MRI follow-up of herpes simplex virus (type 1) radiculomyelitis Neurology, March 1, 1999; 52(5): 1102 - 1102. [Full Text]
|
|
|
|
|
|
H. Kimura, M. Morita, Y. Yabuta, K. Kuzushima, K. Kato, S. Kojima, T. Matsuyama, and T. Morishima Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay J. Clin. Microbiol., January 1, 1999; 37(1): 132 - 136. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
