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Clinical Chemistry 43: 1885-1890, 1997;
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(Clinical Chemistry. 1997;43:1885-1890.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Evaluation of precipitation and direct methods for HDL-cholesterol assay by HPLC

Mitsuyo Okazakia, Keiko Sasamoto, Toshio Muramatsu and Seijin Hosaki1

Laboratory of Chemistry, College of Liberal Arts and Sciences, Tokyo Medical and Dental University, 2-8-30 Kohnodai, Ichikawa-shi, Chiba 272, Japan.
1 School of Allied Health Science, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113, Japan.
a Author for correspondence. Fax 81-473-74-0630; e-mail okazaki.cul{at}cul.tmd.ac.jp

HDL-cholesterol (HDL-C) values measured by precipitation (sodium phosphotungstate–MgCl2) and direct methods were compared with those obtained by HPLC with a new column (TSKgel Lipopropak) and an eluent (TSKeluent LP-1). The HDL-C values determined by the precipitation method were significantly (P <0.001) lower than those by the HPLC method, whereas the HDL-C values by the direct method were slightly but significantly higher (P <0.02) than those by the HPLC method. A quantitative HPLC analysis of the cholesterol concentration in HDL and non-HDL fractions in the supernatant of serum separated by precipitation reagents with different MgCl2 concentrations ranging from 7.3 to 44 mmol/L revealed that reagents with >22 mmol/L MgCl2 precipitated part of HDL as well as non-HDL lipoproteins. The HPLC method providing quantitative and qualitative information with high precision was regarded as being a reliable approach for HDL-C assay. The HPLC can be also used to evaluate alternative methods for cholesterol assay.




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M.-L. Arranz-Pena, J. Tasende-Mata, and F. J. Martin-Gil
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J. M a Simo, I. Castellano, N. Ferre, J. Joven, and J. Camps
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M.-J. Lin, C. Hoke, and B. Ettinger
Evaluation of Homogeneous High-Density Lipoprotein Cholesterol Assay on a BM/Hitachi 747-200 Analyzer
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