Clinical Chemistry
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Clinical Chemistry 43: 1896-1903, 1997;
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(Clinical Chemistry. 1997;43:1896-1903.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Determination of total cholesterol in serum by liquid chromatography–isotope dilution mass spectrometry

Ruediger Kocka, Bert Delvoux and Helmut Greiling

Institute for Clinical Chemistry and Pathobiochemistry, Medical Faculty, University of Technology Aachen, Pauwelsstr. 30, D-52057 Aachen, Germany.
a Author for correspondence. Fax 49-241-88-88-512.

We have developed a liquid chromatography–isotope dilution mass spectrometry procedure to quantify total cholesterol in serum. A particle-beam interface was used for coupling the liquid chromatograph and the mass spectrometer. After electron impact ionization the ions m/z = 386 and m/z = 389 were used for selective ion monitoring of cholesterol and the internal standard [25,26,27-13C]cholesterol. The sample preparation steps required for serum materials are alkaline hydrolysis and an extraction of the cholesterol into the cyclohexane phase. Imprecision for the determination of cholesterol in control materials is typically <1.0%. The deviation from the certified reference values was <0.75% for all control materials tested. A method comparison of the results obtained by this method with those obtained by gas chromatography–isotope dilution mass spectrometry for n = 28 pooled human sera derived from samples analyzed in our routine laboratory did not show differences >2.5%.




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Lipid Res.Home page
D. W. Johnson, H. J. ten Brink, and C. Jakobs
A rapid screening procedure for cholesterol and dehydrocholesterol by electrospray ionization tandem mass spectrometry
J. Lipid Res., October 1, 2001; 42(10): 1699 - 1706.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.