Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 2137-2142, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mario, N.
Right arrow Articles by Giboudeau, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mario, N.
Right arrow Articles by Giboudeau, J.
Related Collections
Right arrow Hematology
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1997;43:2137-2142.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Capillary isoelectric focusing and high-performance cation-exchange chromatography compared for qualitative and quantitative analysis of hemoglobin variants

Nathalie Mario1,a, Bruno Baudin1,2, Christian Aussel1 and Jacqueline Giboudeau1

1 Service de Biochimie A, Hôpital Saint-Antoine, AP-HP, and
2 Laboratoire de Biochimie et Glycobiologie, UFR Pharmacie Université René Descartes-Paris V, Paris, France.
a Address for correspondence: 184 rue du Faubourg Saint-Antoine, 75571 Paris Cedex 12 France. Fax 33 1 49 28 20 77;

We have developed two assays for complete analysis of hemoglobins (Hbs) in the field of hemoglobinopathies: a high-performance cation-exchange liquid chromatography (HPLC) assay on the weak cation-exchanger Poly Cat A and a two-step capillary isoelectric focusing (CIEF) assay on the neutral-coated capillary from Beckman in a narrow pH gradient. The resolution was satisfactory for both HPLC and CIEF and allowed separation of normal and common abnormal Hbs, i.e., Hb A, Hb F, Hb A2, Hb S, Hb C, and Hb E; slight differences were shown for the resolution of unusual variants such as Hb C-Harlem and Hb D-Punjab. The reproducibility of retention times was satisfactory as well for HPLC (CV 3.3%) and CIEF (CV 4.9%). The imprecision of quantification of Hb A2, evaluated at two concentrations, and of Hb F and Hb S was <5%, except for low concentrations of Hb A2quantified by CIEF. Quantitative data obtained for these three Hb forms were highly correlated between the two assays. These results suggest that the new CIEF assay can be competitive with HPLC for complete routine analysis of Hb variants.




The following articles in journals at HighWire Press have cited this article:


Home page
 Annals of Clinical & Laboratory ScienceHome page
L. B. Thomas, S. J. Agosti, M. A. Man, and S. M. Mastorides
Screening for Hemoglobinopathies During Routine Hemoglobin A1c Testing Using the Tosoh G7 Glycohemoglobin Analyzer
Ann. Clin. Lab. Sci., January 1, 2007; 37(3): 251 - 255.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Joutovsky, J. Hadzi-Nesic, and M. A. Nardi
HPLC Retention Time as a Diagnostic Tool for Hemoglobin Variants and Hemoglobinopathies: A Study of 60000 Samples in a Clinical Diagnostic Laboratory
Clin. Chem., October 1, 2004; 50(10): 1736 - 1747.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. M. Clarke and T. N. Higgins
Laboratory Investigation of Hemoglobinopathies and Thalassemias: Review and Update
Clin. Chem., August 1, 2000; 46(8): 1284 - 1290.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. Cotton, C. Lin, B. Fontaine, B. Gulbis, J. Janssens, and F. Vertongen
Evaluation of a Capillary Electrophoresis Method for Routine Determination of Hemoglobins A2 and F
Clin. Chem., February 1, 1999; 45(2): 237 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Mario, B. Baudin, A. Bruneel, J. Janssens, and M. Vaubourdolle
Capillary Zone Electrophoresis for the Diagnosis of Congenital Hemoglobinopathies
Clin. Chem., February 1, 1999; 45(2): 285 - 288.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.