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Secretory carbonic anhydrase isoenzyme (CA VI) in human serum

¹ Department of Anatomy, University of Oulu, Oulu, Finland.

² Parolannummi Garrison Hospital, Finnish Defence Forces, Hattula, Finland.

³ Edward A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, MO.
^a Address correspondence to this author at: Parolannummi Garrison Hospital, P.O. Box 5, FIN-13701 Parolannummi, Finland. Fax 358–3-1814–4612; e-mail jyrki.kivela{at}pp.inet.fi

Carbonic anhydrase VI (CA VI) is a secretory isoenzyme that, by analogy to -amylase, is produced in the salivary glands and delivered into saliva. To determine whether CA VI is transferred into the circulation and is detectable in human serum, we collected blood samples from four healthy subjects at 3-h intervals throughout a 24-h period and measured concentrations of CA VI by a specific time-resolved immunofluorometric assay. All serum samples contained CA VI, the concentrations being ~22 times lower in serum than in the corresponding saliva samples. The presence of CA VI in serum was confirmed by Western blotting, which under reducing conditions identified a 42-kDa polypeptide band corresponding to the monomeric CA VI. The described time-resolved immunofluorometric assay for CA VI might be useful to identify or exclude diseases of the salivary glands in the differential diagnosis of patients whose serum amylase concentrations are increased.

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