Clinical Chemistry
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Clinical Chemistry 43: 2403-2407, 1997;
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(Clinical Chemistry. 1997;43:2403-2407.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Basal concentrations of free and esterified monohydroxylated fatty acids in human blood platelets

Michel Guichardanta, Chantal Thevenon, Jean Francois Pageaux and Michel Lagarde

INSERM. U352, Biochimie et Pharmacologie INSA-Lyon, 20 Ave. Albert Einstein, 69621 Villeurbanne, France.
a Author for correspondence. Fax + 33 4 72 43 85 24; e-mail Michel.Guichardant{at}insa-lyon.fr

Monohydroxylated fatty acids (HO-FA), namely 12-hydroxyeicosatetraenoic and 12-hydroxyheptadecatrienoic acids, are enzymatically formed in response to platelet activation. Different techniques, including gas chromatography (GC) and liquid chromatography–mass spectrometry (LC-MS), have been described to measure HO-FA in activated cells, but they are not well-adapted to resting cells. Measurements of free and esterified HO-FA at basal concentration require the prevention of platelet activation. For this purpose, such an activation was minimized by adding various inhibitors to the anticoagulant. Platelet recovery was greater in the protected group than in controls (473 x 109 ± 4.0 x 109 platelets/L vs 410 x 109 ± 4.53 x 109 platelets/L, respectively) (mean ± SEM, n = 9, P <0.05). Lipids were extracted and immediately hydrogenated to avoid fatty acid autoxidation occurring during the workup. Unesterified and esterified HO-FA were analyzed by GC-MS, and the former were lower in the protected group (1.52 ± 0.84 pmol/109 platelets) than in the unprotected one (12.63 ± 10.52 pmol/109 platelets) (mean ± SEM, n = 9, P <0.05). Interestingly, only traces of HO-FA were detected in both the triglyceride and sterol ester fractions, and they were also weakly esterified in phospholipids.




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Lipid Res.Home page
C. Calzada, E. Vericel, B. Mitel, L. Coulon, and M. Lagarde
12(S)-Hydroperoxy-eicosatetraenoic acid increases arachidonic acid availability in collagen-primed platelets
J. Lipid Res., September 1, 2001; 42(9): 1467 - 1473.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.