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Clinical Chemistry 43: 312-325, 1997;
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(Clinical Chemistry. 1997;43:312-325.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection

Chi-Kong Laia, Ting Lee, Kam-Ming Au and Albert Yan-Wo Chan

Department of Pathology, Princess Margaret Hospital, Lai Chi Kok, Hong Kong.
a Author for correspondence. Fax 852 23700969.

In this HPLC–diode-array detection method for toxicological drug screening, a mixed-mode solid-phase extraction procedure is optimized for isolation of a broad range of drugs from serum and urine. Basic, neutral, and weakly acidic drugs are uniformly recovered. The extract from the solid-phase cartridge is readily injected to a reversed-phase HPLC column for separation by gradient elution. Unknown drugs and metabolites in urine and serum samples from acute drug poisoning cases are rapidly identified by matching their retention times and ultraviolet spectra with hundreds of reference compounds in the library. Urine metabolites of common toxicants from various medications and drugs of abuse are recorded, with their changes of retention times and ultraviolet spectra as related to their metabolic transformations. Glucuronide conjugates of common benzodiazepines, tricyclic antidepressants, and beta-blockers are examined directly without chemical or enzymatic hydrolysis. The system is reliable for diverse clinical investigations of drug overdoses, drug-induced psychoses, and substance abuse.


Key Words: indexing terms: chromatography, reversed-phase • toxicology • abused drugs • drug metabolism and disposition




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Copyright © 1997 by the American Association for Clinical Chemistry.