Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 352-359, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qian, Y.
Right arrow Articles by Lee, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qian, Y.
Right arrow Articles by Lee, C.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 1999;43:352-359.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Two-dimensional gel electrophoresis detects prostate-specific antigen–{alpha}1-antichymotrypsin complex in serum but not in prostatic fluid

Yi Qian1, Julia A. Sensibar1, David J. Zelner1, Anthony J. Schaeffer1, Judith A. Finlay2, Harry G. Rittenhouse2 and Chung Lee1,a

1 Department of Urology, Northwestern University Medical School, Chicago, IL 60611.

2 Cancer Research Program, Hybritech, San Diego, CA 92196.
a Author for correspondence. Fax 312-908-7275; e-mail c-lee7{at}nwu.edu

We investigated the interaction between prostate-specific antigen (PSA) and 1-antichymotrypsin (ACT) in prostatic secretions, identifying PSA and ACT in human serum, prostatic fluid, and seminal plasma by two-dimensional gel electrophoresis (2-D PAGE). Both PSA and ACT were detected in all three body fluids, but PSA-ACT complex was detected only in serum. Moreover, the 2-D PAGE Western blot staining profile for ACT from serum differed from that for prostatic fluid or seminal plasma. Incubation of prostatic fluid with purified ACT led to formation of PSA-ACT complex. Incubation of prostatic fluid with purified PSA, however, failed to form the complex, suggesting that the ACT in prostatic fluid was inactive or inhibited. Given that physiological concentrations of zinc inhibited the formation of PSA-ACT complex, we consider zinc a possible physiological inhibitor of the formation of the PSA-ACT complex. These results indicate that the failure to detect the PSA-ACT complex in prostatic fluid could be related to the inactivation of ACT, the presence of inhibitors (e.g., zinc), or simply the PSA:ACT ratio in the fluid.


Key Words: indexing terms: electrophoresis, polyacrylamide gel • Western blot • prostatic cancer • zinc • complex-formation inhibitors




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
K. Jung, J. Reiche, A. Boehme, C. Stephan, S. A. Loening, D. Schnorr, W. Hoesel, and P. Sinha
Analysis of Subforms of Free Prostate-Specific Antigen in Serum by Two-Dimensional Gel Electrophoresis: Potential to Improve Diagnosis of Prostate Cancer
Clin. Chem., December 1, 2004; 50(12): 2292 - 2301.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. Nurmikko, K. Pettersson, T. Piironen, J. Hugosson, and H. Lilja
Discrimination of Prostate Cancer from Benign Disease by Plasma Measurement of Intact, Free Prostate-specific Antigen Lacking an Internal Cleavage Site at Lys145-Lys146
Clin. Chem., August 1, 2001; 47(8): 1415 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. Nurmikko, V. Vaisanen, T. Piironen, S. Lindgren, H. Lilja, and K. Pettersson
Production and Characterization of Novel Anti-Prostate-specific Antigen (PSA) Monoclonal Antibodies That Do Not Detect Internally Cleaved Lys145-Lys146 Inactive PSA
Clin. Chem., October 1, 2000; 46(10): 1610 - 1618.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Ekins and P. Edwards
Point On the meaning of ""sensitivity""
Clin. Chem., October 1, 1997; 43(10): 1824 - 1831.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.