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Clinical Chemistry 43: 363-368, 1997;
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(Clinical Chemistry. 1997;43:363-368.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Competitive enzyme immunoassay with monoclonal antibody for homovanillic acid measurement in human urine samples

Frédéric Taran, Yveline Frobert, Christophe Créminon, Jacques Grassi, Didier Olichon1, Charles Mioskowski and Philippe Pradellesa

1 Laboratoire CERBA, 95066 Cergy-Pontoise, Cedex 9, France.
a Author for correspondence. Fax (33) 01 69 08 59 07; e-mail pradelles{at}dsvidf.cea.fr

A fast competitive enzyme immunoassay (EIA) for measuring homovanillic acid in human urine samples was developed with a monoclonal antibody and acetylcholinesterase as enzyme label. Enzyme detection was performed by an easy colorimetric assay. Monoclonal antibodies were screened on the basis of sensitivity, specificity, and correlation studies. EIA has a detection limit of 0.5 µmol/L, a CV <10% in the 1.25–10 µmol/L range, and intra- and interassay CVs of <10%. Cross-reactivity with vanillylmandelic acid was 0.5% and <8% for other structurally related catecholamine metabolites. Parallelism of the EIA was shown in dilution studies and the correlation with routine HPLC assay in 62 normal and pathologic samples was EIA = 1.492 (HPLC) - 3.46, Sy|x = 47.52, range = 4–1800 µmol/L, r2 = 0.977. Additional data concerning the validity of this assay were provided by HPLC analysis of urinary immunoreactive material.


Key Words: indexing terms: catecholamines • neuroblastoma • vanillylmandelic acid




The following articles in journals at HighWire Press have cited this article:


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R. Z. Shi, Y.-P. Ho, J. H. K. Yeung, P. M. Y. Or, K. K. W. To, M. W. M. Lau, and M. Arumanayagam
Development of an enzyme-linked immunosorbent assay with monoclonal antibody for quantification of homovanillic in human urine samples
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R. Ekins and P. Edwards
Point On the meaning of ""sensitivity""
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G. A. Smythe, M. W. Duncan, J. Grassi, and P. Pradelles
Immunoassay of Catecholamines and Metabolites • Two of the authors of the article referred to reply:
Clin. Chem., October 1, 1997; 43(10): 2011 - 2012.
[Full Text]


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