Clinical Chemistry
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Clinical Chemistry 43: 390-393, 1997;
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Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 1999;43:390-393.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Comparison of methods for measurement of apolipoprotein B and cholesterol in low-density lipoproteins

Ljubica Vrga, Christine Contacos, Stephen C. H. Li and David R. Sullivana

Department of Clinical Biochemistry, Royal Prince Alfred Hospital, Missenden Rd., Camperdown, New South Wales, 2050, Australia.
1 Nonstandard abbreviations: LDL-C, LDL cholesterol; CAD, coronary artery disease; apo, apolipoprotein; TG, triglyceride; TC, total cholesterol; and AQAP, Australian Quality Assurance Programme.
a Author for correspondence. Fax 61-2-95157931; e-mail davids{at}bioc.rpa.cs.nsw.gov.au

We describe a new method for the direct measurement of LDL-apolipoprotein (apo) B by using a commercial kit that isolates LDL by immunoseparation. We evaluated immunoseparation of LDL for apo B and cholesterol measurement in 46 dyslipidemic patients with LDL-cholesterol (LDL-C) between 1.5 and 8.2 mmol/L, 11 of whom had plasma triglyceride (TG) concentrations >4.0 mmol/L. There was a reasonable correlation (r = 0.94, n = 40) between LDL-apo B obtained after immunoseparation and d >1.006 kg/L apo B obtained after ultracentrifugation. LDL-C by the immunoseparation method also correlated well (r = 0.98, n = 46) with the d >1.006 kg/L cholesterol after ultracentrifugation. These results show that immunoseparation can be used to determine LDL-apo B, even in hypertriglyceridemic samples. This method may provide a quick and simple alternative for the identification of hyperapobetalipoproteinemia, even when TG concentrations are high.


Key Words: indexing terms: ultracentrifugation • triglycerides • precipitin tests • immunologic techniques




The following articles in journals at HighWire Press have cited this article:


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J. R. Burnett and G. F. Watts
Estimating LDL ApoB: Infomania or Clinical Advance?
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M. Nauck, G. R. Warnick, and N. Rifai
Methods for Measurement of LDL-Cholesterol: A Critical Assessment of Direct Measurement by Homogeneous Assays versus Calculation
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F. Gomez, J. Camps, J. M. Simo, N. Ferre, and J. Joven
Agreement Study of Methods Based on the Elimination Principle for the Measurement of LDL- and HDL-Cholesterol Compared with Ultracentrifugation in Patients with Liver Cirrhosis
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