| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Address correspondence to this author, at: Department of Urology, Mail Stop 356510, University of Washington, Seattle, WA 98195. Fax 206-543-1146; e-mail ecorey{at}u.washington.edu
The possibility of improving diagnosis of micrometastases from prostate cancer by further enhancing the detection of prostate-specific antigen-producing cells in circulation is being evaluated. We have developed a reverse transcriptase-PCR protocol with the desirable characteristics of low limit of detection, high specificity, reproducibility of response, and ease of performance. Among the procedural alterations that have contributed to these improvements are longer PCR primers, a two-step amplification cycle, and hot-start PCR. We have lowered the limit of detection to one LNCaP prostate-cancer cell in 108 peripheral blood mononuclear cells, and samples of blood and bone marrow from healthy donors have yielded no false positives. Because PCR procedures frequently exhibit tube-to-tube variability, we have incorporated a set of internal and external controls into the protocol—a significant advance in assuring assay reliability.
Key Words: indexing terms: cancer • metastasis • peripheral blood mononuclear cells
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  D. Schamhart, J. Swinnen, K.-H. Kurth, A. Westerhof, R. Kusters, H. Borchers, and C. Sternberg Numeric Definition of the Clinical Performance of the Nested Reverse Transcription-PCR for Detection of Hematogenous Epithelial Cells and Correction for Specific mRNA of Non-Target Cell Origin as Evaluated for Prostate Cancer Cells Clin. Chem., September 1, 2003; 49(9): 1458 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Grammatopoulos, Y Elliott, S C Smith, I Brown, R J Grieve, E W Hillhouse, M A Levine, and M D Ringel Measurement of thyroglobulin mRNA in peripheral blood as an adjunctive test for monitoring thyroid cancer Mol. Pathol., June 1, 2003; 56(3): 162 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ylikoski, K. Pettersson, J. Nurmi, K. Irjala, M. Karp, H. Lilja, T. Lovgren, and M. Nurmi Simultaneous Quantification of Prostate-specific Antigen and Human Glandular Kallikrein 2 mRNA in Blood Samples from Patients with Prostate Cancer and Benign Disease Clin. Chem., August 1, 2002; 48(8): 1265 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Corey, J. E. Quinn, M. J. Emond, K. R. Buhler, L. G. Brown, and R. L. Vessella Inhibition of Androgen-independent Growth of Prostate Cancer Xenografts by 17{beta}-Estradiol Clin. Cancer Res., April 1, 2002; 8(4): 1003 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ylikoski, M. Karp, K. Pettersson, H. Lilja, and T. Lovgren Simultaneous Quantification of Human Glandular Kallikrein 2 and Prostate-Specific Antigen mRNAs in Peripheral Blood from Prostate Cancer Patients J. Mol. Diagn., August 1, 2001; 3(3): 111 - 122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Malatesta, F. Mannello, F. Luchetti, F. Marcheggiani, L. Condemi, S. Papa, and G. Gazzanelli Prostate-Specific Antigen Synthesis and Secretion by Human Placenta: A Physiological Kallikrein Source during Pregnancy J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 317 - 321. [Abstract] [Full Text]
|
|
|
|
|
|
M. D. Ringel, P. L. Balducci-Silano, J. S. Anderson, C. A. Spencer, J. Silverman, Y. H. Sparling, G. L. Francis, K. D. Burman, L. Wartofsky, P. W. Ladenson, et al. Quantitative Reverse Transcription-Polymerase Chain Reaction of Circulating Thyroglobulin Messenger Ribonucleic Acid for Monitoring Patients with Thyroid Carcinoma J. Clin. Endocrinol. Metab., November 1, 1999; 84(11): 4037 - 4042. [Abstract] [Full Text]
|
|
|
|
|
|
A. Ylikoski, M. Sjoroos, A. Lundwall, M. Karp, T. Lovgren, H. Lilja, and A. Iitia Quantitative Reverse Transcription-PCR Assay with an Internal Standard for the Detection of Prostate-specific Antigen mRNA Clin. Chem., September 1, 1999; 45(9): 1397 - 1407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Cher, J. G. de Oliveira, A. A. Beaman, J. A. Nemeth, M. Hussain, and D. P. Wood Jr. Cellular Proliferation and Prevalence of Micrometastatic Cells in the Bone Marrow of Patients with Clinically Localized Prostate Cancer Clin. Cancer Res., September 1, 1999; 5(9): 2421 - 2425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mannello, M. Malatesta, F. Luchetti, S. Papa, S. Battistelli, and G. Gazzanelli Immunoreactivity, Ultrastructural Localization, and Transcript Expression of Prostate-specific Antigen in Human Neuroblastoma Cell Lines Clin. Chem., January 1, 1999; 45(1): 78 - 84. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Ringel, P. W. Ladenson, and M. A. Levine Molecular Diagnosis of Residual and Recurrent Thyroid Cancer by Amplification of Thyroglobulin Messenger Ribonucleic Acid in Peripheral Blood J. Clin. Endocrinol. Metab., December 1, 1998; 83(12): 4435 - 4442. [Abstract] [Full Text]
|
|
|
|
|
|
F. Mannello, F. Luchetti, D. Lancioli, S. Battistelli, S. Papa, and G. Gazzanelli Prostate-specific Antigen Expression in Neoplastic Human Myeloid Cell Lines Clin. Chem., September 1, 1998; 44(9): 1991 - 1993. [Full Text] [PDF]
|
|
|
|
|
|
M. Verhaegen, P. C. Ioannou, and T. K. Christopoulos Quantification of prostate-specific antigen mRNA by coamplification with a recombinant RNA internal standard and microtiterwell-based hybridization Clin. Chem., June 1, 1998; 44(6): 1170 - 1176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Liu, L. D. True, L. LaTray, P. S. Nelson, W. J. Ellis, R. L. Vessella, P. H. Lange, L. Hood, and G. van den Engh Cell-cell interaction in prostate gene regulation and cytodifferentiation PNAS, September 30, 1997; 94(20): 10705 - 10710. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
