Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 467-475, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (41)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishikawa, Y.
Right arrow Articles by Roberts, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishikawa, Y.
Right arrow Articles by Roberts, R.
Related Collections
Right arrow Evidence Based Laboratory Medicine and Test Utilization
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 1997;43:467-475.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Reversible myocardial ischemic injury is not associated with increased creatine kinase activity in plasma

Yuichi Ishikawa1, Jeffrey E. Saffitz2, Terrence L. Mealman2, Ann M. Grace2 and Robert Roberts3,a

1 Department of Medicine, University of Kobe, Kobe, Japan.

2 Departments of Pathology and Cardiology, Washington University, St. Louis, MO.

3 Department of Medicine, Section of Cardiology, Baylor College of Medicine, 6550 Fannin, MS SM677, Houston, TX 77030.
a Author for correspondence. Fax 713-790-4348; e-mail dweaver{at}bmc.tmc.edu

Creatine kinase (CK) isoenzymes MM, MB, and BB are located primarily in the cell cytosol, and increased CKMB in plasma is the hallmark of myocardial infarction. However, whether CK is released with reversible ischemic injury remains controversial. Here, we assessed plasma CK activity—cytosolic and mitochondrial CK—in serial samples (every 10 min for 60 min, then hourly or every 4 h for 48 h) from 46 conscious dogs after transient or sustained coronary occlusion. Four dogs were sham-operated (controls); four underwent sustained coronary occlusion (96 h); and 38 underwent transient coronary occlusion (10–40 min) followed by 48 h of reperfusion. In postmortem histological examination of the dogs' hearts by light and electron microscopy, we looked for ischemia or necrosis. The presence of cell swelling and glycogen depletion was indicative of ischemia, whereas the added presence of cell disruption indicated necrosis. Coronary occlusion for >=20 min consistently increased plasma mitochondrial and total CK activity and produced histologically evident myocardial necrosis. In contrast, after 10 to 15 min of coronary occlusion, 12 of 14 animals, despite extensive severe reversible ischemia, showed no increase in plasma CK; the remaining 2, which had increased plasma CK, had subendocardial necrosis. Thus, cytosolic or mitochondrial CK is released from the heart only when there has been irreversible myocardial injury—a finding with significant diagnostic and therapeutic implications.


Key Words: indexing terms: mitochondrial enzymes • coronary occlusion • dogs • heart damage • radioimmunoassay




The following articles in journals at HighWire Press have cited this article:


Home page
 Toxicol PatholHome page
D. B. Walker
Serum Chemical Biomarkers of Cardiac Injury for Nonclinical Safety Testing
Toxicol Pathol, January 1, 2006; 34(1): 94 - 104.
[Abstract] [Full Text] [PDF]

Home page
 CMAJHome page
L. Babuin and A. S. Jaffe
Troponin: the biomarker of choice for the detection of cardiac injury
Can. Med. Assoc. J., November 8, 2005; 173(10): 1191 - 1202.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
K. Arakawa, Y. Kawai, T. Kumamoto, N. Morikawa, M. Yoshida, H. Tada, R. Kawaguchi, K. Taniguchi, I. Miyamori, Y. Kominato, et al.
Serum deoxyribonuclease I activity can be used as a sensitive marker for detection of transient myocardial ischaemia induced by percutaneous coronary intervention
Eur. Heart J., November 2, 2005; 26(22): 2375 - 2380.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Liu, B. Xu, T. A. Cavalieri, and C. E. Hock
Attenuation of antioxidative capacity enhances reperfusion injury in aged rat myocardium after MI/R
Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2719 - H2727.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
N. S. Kleiman, N. Lakkis, C. P. Cannon, S. A. Murphy, P. M. DiBattiste, L. A. Demopoulos, W. S. Weintraub, E. Braunwald, and TACTICS-TIMI 18 Investigators
Prospective analysis of creatine kinase muscle-brain fraction and comparison with troponin T to predict cardiac risk and benefit of an invasive strategy in patients with non-ST-elevation acute coronary syndromes
J. Am. Coll. Cardiol., September 18, 2002; 40(6): 1044 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. S. Jaffe, J. Ravkilde, R. Roberts, U. Naslund, F. S. Apple, M. Galvani, and H. Katus
It's Time for a Change to a Troponin Standard
Circulation, September 12, 2000; 102(11): 1216 - 1220.
[Full Text] [PDF]

Home page
 Eur Heart JHome page
Myocardial
Myocardial infarction redefined--A consensus document of The Joint European Society of Cardiology/American College of Cardiology Committee for the Redefinition of Myocardial Infarction
Eur. Heart J., September 2, 2000; 21(18): 1502 - 1513.
[Abstract] [PDF]

Home page
 Physiol. Rev.Home page
M. Wyss and R. Kaddurah-Daouk
Creatine and Creatinine Metabolism
Physiol Rev, July 1, 2000; 80(3): 1107 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
J. Zimmerman, R. Fromm, D. Meyer, A. Boudreaux, C.-C. C. Wun, R. Smalling, B. Davis, G. Habib, and R. Roberts
Diagnostic Marker Cooperative Study for the Diagnosis of Myocardial Infarction
Circulation, April 6, 1999; 99(13): 1671 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
R. Roberts and R. E. Fromm
Management of Acute Coronary Syndromes Based on Risk Stratification by Biochemical Markers : An Idea Whose Time Has Come
Circulation, November 3, 1998; 98(18): 1831 - 1833.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.