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Articles |
1
Institut für Klinische Chemie and
2
Klinik für Psychiatrie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany
a Author for correspondence. Fax 0451/5002904.
The current HPLC method for the determination of carbohydrate-deficient transferrin (CDT) yields ratios of CDT isoforms in relation to total transferrin, whereas the use of absolute concentrations obtainable in routine analysis by RIA and the reference ranges based hereupon is more convenient. We describe a modified HPLC method that likewise gives absolute CDT concentrations by using a calibrator prepared by treatment of transferrin with neuraminidase. Separation of isoforms could be improved and analysis time reduced to ~2 h. Iron saturation proved stable during chromatography. In contrast to a commercial RIA, the cheaper and more time-saving HPLC method excludes erroneous results caused by aged samples or genetic transferrin variants and enables the determination of asialo- and disialotransferrin. Both methods showed comparable precision and correlated with each other (y = 1.76 + 0.27x; Sy|x = 5.38); for the HPLC method precision was 1.3–9.8% (within assay) and 6.2–10.6% (between assay). The clinical evaluation with a cutoff concentration of 80 mg/L resulted in a diagnostic specificity of 100% and a sensitivity of 82.5%.
Key Words: indexing terms: desialylated transferrin • neuraminidase • alcohol abuse marker • high-performance liquid chromatography • radioimmunoassay • isoelectric focusing
The following articles in journals at HighWire Press have cited this article:
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  A. Helander, A. Husa, and J.-O. Jeppsson Improved HPLC Method for Carbohydrate-deficient Transferrin in Serum Clin. Chem., November 1, 2003; 49(11): 1881 - 1890. [Abstract] [Full Text] [PDF]
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F. J. Legros, V. Nuyens, M. Baudoux, K. Zouaoui Boudjeltia, J.-L. Ruelle, J. Colicis, F. Cantraine, and J.-P. Henry Use of Capillary Zone Electrophoresis for Differentiating Excessive from Moderate Alcohol Consumption Clin. Chem., March 1, 2003; 49(3): 440 - 449. [Abstract] [Full Text] [PDF]
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A. Helander, G. Eriksson, H. Stibler, and J.-O. Jeppsson Interference of Transferrin Isoform Types with Carbohydrate-deficient Transferrin Quantification in the Identification of Alcohol Abuse Clin. Chem., July 1, 2001; 47(7): 1225 - 1233. [Abstract] [Full Text] [PDF]
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J. M. Lacey, H. R. Bergen, M. J. Magera, S. Naylor, and J. F. O'Brien Rapid Determination of Transferrin Isoforms by Immunoaffinity Liquid Chromatography and Electrospray Mass Spectrometry Clin. Chem., March 1, 2001; 47(3): 513 - 518. [Abstract] [Full Text] [PDF]
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T. Arndt Carbohydrate-deficient Transferrin as a Marker of Chronic Alcohol Abuse: A Critical Review of Preanalysis, Analysis, and Interpretation Clin. Chem., January 1, 2001; 47(1): 13 - 27. [Abstract] [Full Text] [PDF]
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L. Dibbelt Does Trisialo-Transferrin Provide Valuable Information for the Laboratory Diagnosis of Chronically Increased Alcohol Consumption by Determination of Carbohydrate-deficient Transferrin? Clin. Chem., August 1, 2000; 46(8): 1203 - 1205. [Full Text] [PDF]
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R. Hackler, T. Arndt, A. Helwig-Rolig, J. Kropf, A. Steinmetz, and J. R. Schaefer Investigation by Isoelectric Focusing of the Initial Carbohydrate-deficient Transferrin (CDT) and non-CDT Transferrin Isoform Fractionation Step Involved in Determination of CDT by the ChronAlcoI.D. Assay Clin. Chem., April 1, 2000; 46(4): 483 - 492. [Abstract] [Full Text] [PDF]
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T. Arndt, R. Hackler, T. O. Kleine, and A. M. Gressner Validation by isoelectric focusing of the anion-exchange isotransferrin fractionation step involved in determination of carbohydrate-deficient transferrin by the CDTect assay Clin. Chem., January 1, 1998; 44(1): 27 - 34. [Abstract] [Full Text] [PDF]
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