Clinical Chemistry Siemens Point of Care - Urinalysis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 533-538, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (23)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Etchison, J. R.
Right arrow Articles by Freeze, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Etchison, J. R.
Right arrow Articles by Freeze, H. H.
Related Collections
Right arrow General Clinical Chemistry
(Clinical Chemistry. 1997;43:533-538.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Enzymatic assay of D-mannose in serum

James R. Etchison and Hudson H. Freezea

The Burnham Institute, Glycobiology/Carbohydrate Chemistry Program, 10901 North Torrey Pines Rd., La Jolla, CA 92037
a Author for correspondence: Fax 619-646-3193; email hudson{at}ljcrf.edu

We describe a new and improved enzymatic assay for determining the concentration of D-mannose in sera. Serum D-glucose is selectively converted to glucose-6 phosphate with the highly specific thermostable glucokinase (EC 2.7.1.2) from Bacillus stearothermophilus. The anionic reaction products and excess substrates are removed by a rapid and simple anion-exchange chromatography step in microcentrifuge spin columns. D-Mannose in the glucose-depleted sample is then assayed spectrophotometrically by using coupled enzymatic reactions. The quantitative elimination of glucose from the serum samples allowed the accurate and reproducible assay of serum mannose in the 0–200 µmol/L range. Recovery of mannose added to serum (5–200 µmol/L) was 94% ± 4.4%. The intraassay CV was 6.7% at 40 µmol/L mannose (n = 5; 39.6 ± 1.6 µmol/L) and 4.4% at 80 µmol/L (n = 11; 75.0 ± 1.8 µmol/L); the interassay CV at these concentrations was 12.2% (n = 7; 36.9 ± 2.1 µmol/L) and 9.8% (n = 7; 74.2 ± 2.7 µmol/L), respectively. Sera from 11 healthy human volunteers contained an average of 54.1 ± 11.9 µmol/L mannose (range 36–81 µmol/L).


Key Words: indexing terms: glucose oxidase • glucokinase • carbohydrate-deficient glycoprotein syndrome • phosphomannose isomerase • phosphoglucose isomerase




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Bacteriol.Home page
A. Angelov, M. Putyrski, and W. Liebl
Molecular and Biochemical Characterization of {alpha}-Glucosidase and {alpha}-Mannosidase and Their Clustered Genes from the Thermoacidophilic Archaeon Picrophilus torridus.
J. Bacteriol., October 1, 2006; 188(20): 7123 - 7131.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. Taguchi, E. Yamashita, T. Mizutani, H. Nakajima, M. Yabuuchi, N. Asano, and I. Miwa
Hepatic glycogen breakdown is implicated in the maintenance of plasma mannose concentration
Am J Physiol Endocrinol Metab, March 1, 2005; 288(3): E534 - E540.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Taguchi, I. Miwa, T. Mizutani, H. Nakajima, Y. Fukumura, I. Kobayashi, M. Yabuuchi, and I. Miwa
Determination of D-Mannose in Plasma by HPLC
Clin. Chem., January 1, 2003; 49(1): 181 - 183.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
H. A. Carchon and J. Jaeken
Determination of D-Mannose in Serum by Capillary Electrophoresis
Clin. Chem., July 1, 2001; 47(7): 1319 - 1321.
[Full Text] [PDF]

Home page
 J. Med. Genet.Home page
P de Lonlay, N Seta, S Barrot, B Chabrol, V Drouin, B M Gabriel, H Journel, M Kretz, J Laurent, M Le Merrer, et al.
A broad spectrum of clinical presentations in congenital disorders of glycosylation I: a series of 26 cases
J. Med. Genet., January 1, 2001; 38(1): 14 - 19.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K. Panneerselvam, J. R. Etchison, and H. H. Freeze
Human Fibroblasts Prefer Mannose over Glucose as a Source of Mannose for N-Glycosylation. EVIDENCE FOR THE FUNCTIONAL IMPORTANCE OF TRANSPORTED MANNOSE
J. Biol. Chem., September 12, 1997; 272(37): 23123 - 23129.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.