Clinical Chemistry
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Clinical Chemistry 43: 575-584, 1997;
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(Clinical Chemistry. 1997;43:575-584.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Prostate-specific antigen: characterization of epitopes by synthetic peptide mapping and inhibition studies

Eva Coreya, Sandra K. Wegner, Michael J. Corey and Robert L. Vessella

Tumor Immunology Laboratory of the Urology Department, School of Medicine of the University of Washington, Seattle, WA 98195.
a Address correspondence to this author at: Mailstop 356510, Department of Urology, University of Washington, Seattle, WA 98195. Fax 206-543-1146; e-mail ecorey{at}u.washington.edu

To improve our understanding of the prostate-specific antigen (PSA) antigenic regions, we studied the association targets of one anti-PSA polyclonal antibody and 10 anti-PSA monoclonal antibodies (mAbs). We also examined the ability of the mAbs to inhibit PSA enzymatic activity and block the association of PSA with {alpha}1-antichymotrypsin (ACT). Linear epitope mapping with a polyclonal antibody indicated the presence of six major antigenic regions in PSA. Examination of the panel of mAbs established that three of them bind to linear epitopes. Five of the mAbs inhibited >90% of PSA enzymatic activity. However, inhibition of PSA enzymatic activity and hindrance of PSA-ACT association by mAbs cannot be used to predict whether the mAbs bind to free PSA, the PSA-ACT complex, or both. Some of the mAbs may block PSA-ACT association through peripheral occlusion of the binding site, or through induction of conformational changes in PSA.


Key Words: indexing terms: linear epitope • {alpha}1-antichymotrypsin • proteolytic activity




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J. Leinonen, P. Wu, and U.-H. Stenman
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S. Michel, G. Deleage, J.-P. Charrier, J. Passagot, N. Battail-Poirot, G. Sibai, M. Jolivet, and C. Jolivet-Reynaud
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M. H. Black, C. L. Grass, J. Leinonen, U.-H. Stenman, and E. P. Diamandis
Characterization of Monoclonal Antibodies for Prostate-specific Antigen and Development of Highly Sensitive Free Prostate-specific Antigen Assays
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