Clinical Chemistry
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Clinical Chemistry 43: 627-634, 1997;
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(Clinical Chemistry. 1997;43:627-634.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Quantitative colorimetric determination of urinary p-aminophenol with an automated analyzer

Jan F. Van Bocxlaer, Karine M. Clauwaert, Willy E. Lambert and André P. De Leenheera

Laboratorium voor Toxicologie, Universiteit Gent, Harelbekestraat 72, B-9000 Gent, Belgium
a Author for correspondence. Fax 32.9.264 81 97; e-mail Andre.DeLeenheer{at}rug.ac.be

We developed an automated colorimetric method for the quantitative determination of p-aminophenol with a Cobas Mira analyzer. The procedure can be used for the biological monitoring of human exposure to aniline. An absorbed aniline dose is extensively oxidized to p-aminophenol, which is excreted in urine mainly as glucurono- and sulfo- conjugates. After enzymatic hydrolysis, we reacted the free compound with resorcinol in the presence of manganese ions to form an indophenol dye, which is measured at 550 nm. Excellent accuracy (102.8%, 103.9%, and 96.8% at 2.5, 50, and 90 mg/L, respectively) and precision (7.7%, 2.1%, and 0.8% CV for within-run and 11.1%, 4.7%, and 4.6% for total reproducibility at 2.5, 50, and 90 mg/L, respectively) were achieved over a linear concentration range of 2.0 to 100 mg/L. The detection limit was 0.9 mg/L and no significant interference (except for o-aminophenol) was found for several investigated drugs and related compounds. The proposed method was used for a stability study and to analyze several samples from an occupational health screen.


Key Words: indexing terms: 4-aminophenol • occupational medicine • aniline • stability




The following articles in journals at HighWire Press have cited this article:


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 Annals of Clinical & Laboratory ScienceHome page
C.-F. Chen, Y.-T. Tseng, H.-K. Tseng, and T.-Z. Liu
Automated Spectrophotometric Assay for Urine p-Aminophenol by an Oxidative Coupling Reaction
Ann. Clin. Lab. Sci., July 1, 2004; 34(3): 336 - 340.
[Abstract] [Full Text] [PDF]


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