Clinical Chemistry
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Clinical Chemistry 43: 638-643, 1997;
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(Clinical Chemistry. 1997;43:638-643.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Development of a urodilatin-specific antibody and radioimmunoassay for urodilatin in human urine

Jan Carstens1,a, Kaare T. Jensen1, Per Ivarsen1, Lars M. Rasmussen2 and Erling B. Pedersen1

1 Research Laboratory of Nephrology and Hypertension, Aarhus University Hospital, Skejby Hospital, Aarhus 8200, Denmark.

2 Research Laboratory of Molecular Pathology, Institute of Pathology, Aarhus Kommunehospital, Aarhus, Denmark.
a Author for correspondence. Fax +45 89 49 60 03.

Urodilatin (URO) (95–126) is a renal-derived natriuretic peptide that is isolated only from human urine. This study describes the development of a URO-specific antibody and a RIA for URO in urine. At present, there is no commonly available URO-specific antibody. We produced a URO-specific antibody without cross-reactivity with atrial natriuretic peptide (ANP) analogs by immunization of rabbits with the URO (95–126) peptide and subsequent purification of the resulting URO antiserum with affinity chromatography with CNBr-activated Sepharose 4B. The urine samples were ethanol-extracted before assay. The CVs were 6.7% (intraassay) and 14.1% (interassay). This study reports the circadian urinary excretion of URO in 24 healthy subjects with seven sampling periods per 24 h.


Key Words: indexing terms: atrial natriuretic peptide • sodium • diuresis • circadian rhythm




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