Clinical Chemistry
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Clinical Chemistry 43: 644-648, 1997;
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(Clinical Chemistry. 1997;43:644-648.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Capillary electrophoresis system for hemoglobin A1c determinations evaluated

Cees J. A. Doelman1,a, Carla W. M. Siebelder1, Wim A. Nijhof1, Cas W. Weykamp1, Jacques Janssens2 and Theo J. Penders1

1 Department of Clinical Chemistry and Hematology, Queen Beatrix Hospital, Beatrixpark 1, 7101 BN Winterswijk, The Netherlands.

2 Analis S.A., 14 Rue Dewez, B 5000 Namur, Belgium.
a Author for correspondence. Fax +31543524265.

Hb A1c is the analyte of choice for monitoring metabolic control in patients with diabetes mellitus. Here we present a new analytical technique for measuring Hb A1c, capillary electrophoresis. The Hb A1c determination is not influenced by the labile Hb A1c fraction or by carbamylated or acetylated hemoglobin derivatives. Also, hemoglobin variants (Hb F, Hb S, and Hb C) do not interfere. This new application of capillary electrophoresis seems to be a valuable analytical tool for measuring Hb A1c in the clinical laboratory.


Key Words: indexing terms: diabetes mellitus • glycohemoglobin • hemoglobin variants




The following articles in journals at HighWire Press have cited this article:


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C. Gay-Bellile, D. Bengoufa, P. Houze, D. Le Carrer, M. Benlakehal, B. Bousquet, B. Gourmel, and T. Le Bricon
Automated Multicapillary Electrophoresis for Analysis of Human Serum Proteins
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C. W. Weykamp, K. Miedema, T. de Haan, and C. J.A. Doelman
Carbamylated Hemoglobin Interference in Glycohemoglobin Assays
Clin. Chem., March 1, 1999; 45(3): 438 - 440.
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F. Cotton, C. Lin, B. Fontaine, B. Gulbis, J. Janssens, and F. Vertongen
Evaluation of a Capillary Electrophoresis Method for Routine Determination of Hemoglobins A2 and F
Clin. Chem., February 1, 1999; 45(2): 237 - 243.
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N. Mario, B. Baudin, A. Bruneel, J. Janssens, and M. Vaubourdolle
Capillary Zone Electrophoresis for the Diagnosis of Congenital Hemoglobinopathies
Clin. Chem., February 1, 1999; 45(2): 285 - 288.
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S. Blincko and R. Edwards
Non-separation assay for glycohemoglobin
Clin. Chem., June 1, 1998; 44(6): 1302 - 1308.
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