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Clinical Chemistry 43: 669-674, 1997;
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(Clinical Chemistry. 1997;43:669-674.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Automated measurement of mouse apolipoprotein B: convenient screening tool for mouse models of atherosclerosis

Daniel M. Levine1,a and Kevin Jon Williams2

1 The Rogosin Institute and Department of Biochemistry, The New York Hospital–Cornell Medical Center, 505 East 70th St., New York, NY 10021.

2 The Dorrance H. Hamilton Research Laboratories, Division of Endocrinology, Diabetes, and Metabolic Diseases, Department of Medicine, Jefferson Medical College of Thomas Jefferson University, 1020 Locust St., Suite 349, Philadelphia, PA 19107-6799.
a Author for correspondence. Fax 212-327-7331; e-mail dmlevine{at}mail.med.cornell.edu

Although mice are commonly used for studies of atherosclerosis, investigators have had no convenient way to quantify apolipoprotein (apo) B, the major protein of atherogenic lipoproteins, in this model. We now report an automated immunoturbidimetric assay for mouse apo B with an NCCLS imprecision study CV <5%. Added hemoglobin up to 50 g/L did not interfere with the assay, nor did one freeze–thaw cycle of serum samples. Assay linearity extends to apo B concentrations of 325 mg/L. We have used the assay to determine serum apo B concentrations under several atherogenic conditions, including the apo E "knock-out" genotype and treatment with a high-cholesterol diet. Our assay can be used to survey inbred mouse strains for variants in apo B concentration or regulation. Moreover, the mouse can now be used as a convenient small-animal model to screen compounds that may lower apo B concentrations.


Key Words: indexing terms: animal models of disease • apo E • LDL receptor • diet, effects of • lipoproteins • mice, transgenic




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