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Clinical Chemistry 43: 771-778, 1997;
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(Clinical Chemistry. 1997;43:771-778.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Potential of electrospray mass spectrometry for quantifying glycohemoglobin

Norman B. Roberts1,a, Brian N. Green2 and Michael Morris2

1 Department of Clinical Chemistry, Royal Liverpool University Hospital, PO Box 147, Liverpool L69 3BX, UK.

2 Micromass UK Ltd., Tudor Road, Altrincham, Cheshire WA14 5RZ, UK.
a Author for correspondence. Fax 0161-282-4400; e-mail mike.morris{at}micromass.co.uk

An electrospray ionization–mass spectrometric procedure has been developed for determining glycohemoglobin. Whole-blood samples from 78 diabetic and 50 nondiabetic subjects (glycation range 3–15%, as determined by electrospray mass spectrometry) were diluted 500-fold in an acidic denaturing solvent and introduced directly into a mass spectrometer. The resulting mass spectra were then processed to estimate the percentage of glycohemoglobin present in the sample. Total analysis time, including plotting the spectra and computing the percentage of glycation, was ~3 min. The imprecision (CV) of the method was <5.1% for inter- and intrabatch analyses for total glycohemoglobin in the range 3.6–14%. Comparison of the mass spectrometric results with those from established affinity chromatographic procedures showed good overall agreement. The relative glycation of the {alpha}- and ß-chains was determined directly and was shown to be constant (0.64:1) over the glycation range measured. Only single glucose attachment to both the {alpha}- and ß-chains was observed.


Key Words: indexing terms: diabetes • method comparison




The following articles in journals at HighWire Press have cited this article:


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Electrospray Ionization Mass Spectrometric Analysis of the Globin Chains in Hemoglobin Heterozygotes Can Detect the Variants HbC, D, and E
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F. Q. Nuttall
Evidence for Independent Heritability of the Glycation Gap (Glycosylation Gap) Fraction of HbA1c in Nondiabetic Twins: Response to Cohen et al.
Diabetes Care, April 1, 2007; 30(4): e14 - e14.
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U. Krishnamurti and M. W. Steffes
Glycohemoglobin: A Primary Predictor of the Development or Reversal of Complications of Diabetes Mellitus
Clin. Chem., July 1, 2001; 47(7): 1157 - 1165.
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L. Bry, P. C. Chen, and D. B. Sacks
Effects of Hemoglobin Variants and Chemically Modified Derivatives on Assays for Glycohemoglobin
Clin. Chem., February 1, 2001; 47(2): 153 - 163.
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N. B. Roberts, A. B. Amara, M. Morris, and B. N. Green
Long-Term Evaluation of Electrospray Ionization Mass Spectrometric Analysis of Glycated Hemoglobin
Clin. Chem., February 1, 2001; 47(2): 316 - 321.
[Abstract] [Full Text] [PDF]

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E. S Kilpatrick
Glycated haemoglobin in the year 2000
J. Clin. Pathol., May 1, 2000; 53(5): 335 - 339.
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A. Lapolla, D. Fedele, M. Plebani, R. Aronica, M. Garbeglio, R. Seraglia, M. D'Alpaos, and P. Traldi
Evaluation of Glycated Globins by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
Clin. Chem., February 1, 1999; 45(2): 288 - 290.
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K. P. Peterson, J. G. Pavlovich, D. Goldstein, R. Little, J. England, and C. M. Peterson
What is hemoglobin A1c? An analysis of glycated hemoglobins by electrospray ionization mass spectrometry
Clin. Chem., September 1, 1998; 44(9): 1951 - 1958.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.