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Clinical Chemistry 43: 801-807, 1997;
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(Clinical Chemistry. 1997;43:801-807.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Automation of human sperm cell analysis by flow cytometry

Fulvio Ferrara1, Rita Daverio1, Giuliano Mazzini2, Pierangelo Bonini1 and Giuseppe Banfi1,a

1 Department of Laboratory Medicine, Scientific Institute H.S. Raffaele, via Olgettina no. 60, 20132, Milan, Italy.

2 Department of Histochemistry, National Research Council, Pavia, Italy.
a Author for correspondence. Fax 39-2-26432640.

Semen sample analysis is routinely performed by microscopical evaluation and manual techniques by laboratory operators; the analysis is affected by a wide imprecision related to variability among observers, influencing its clinical validity. Our aim was to automate sperm analysis with the use of flow cytometry for evaluation of cell counts and typing and with the use of a new membrane-permeant nucleic acid stain for evaluation of sperm viability. Statistical analysis of the comparison between manual and automated methods for sperm counts was performed by the Bland and Altman method; the mean difference was 0.243 x 106 sperms/mL. The precision of the flow cytometric analysis was evaluated with whole sperm; the between-run CV was 7.5% and the within-run CV was 2.5%. Data observed suggest that flow cytometric sperm analysis, with high precision and accuracy and low costs, can be proposed for routine use in clinical laboratories.


Key Words: indexing terms: vital staining • semen analysis




The following articles in journals at HighWire Press have cited this article:


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 Hum ReprodHome page
S. Perticarari, G. Ricci, M. Granzotto, R. Boscolo, C. Pozzobon, S. Guarnieri, A. Sartore, and G. Presani
A new multiparameter flow cytometric method for human semen analysis
Hum. Reprod., February 1, 2007; 22(2): 485 - 494.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Ricci, G. Presani, S. Guaschino, R. Simeone, and S. Perticarari
Leukocyte detection in human semen using flow cytometry
Hum. Reprod., June 1, 2000; 15(6): 1329 - 1337.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.