Clinical Chemistry
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Clinical Chemistry 43: 824-831, 1997;
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(Clinical Chemistry. 1997;43:824-831.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Modified and improved anti-acetylcholine receptor (AChR) antibody assay: comparison of analytical and clinical performance with conventional anti-AChR antibody assay

Bruno Ferrero1,a, Giuseppe Aimo2, Roberto Pagni2, Bruno Bergamasco1, Maria R. Bongioanni1, Lodovico Bergamini1 and Luca Durelli1

1 Department of Neurosciences, and
2 Laboratory "Baldi e Riberi", University Hospital "Molinette", Via Cherasco 15, I-10126, Turin, Italy.
a Author for correspondence. Fax Int +31 11 6963487; e-mail chinigo{at}golgi.molinette.unito.it

We developed a modified anti-acetylcholine receptor (AChR) antibody (Ab) assay based on a radioreceptor assay and a calibration curve. We compared the analytical and clinical performances of this modified assay with those of the conventional anti-AChR Ab radioreceptor assay. Serum specimens were from patients with myasthenia gravis (MG) (n = 156) and from control subjects (n = 106). The modified assay demonstrated lower within-assay (4.0–6.6%) and between-assay (5.3–7.8%) CVs, greater linearity, lower cost, and shorter assay time than the conventional method. ROC curve analysis indicated almost identical specificity and sensitivity (>0.92) for these two anti-AChR Ab assays. The modified and conventional assays were also equivalent for blocking anti-AChR Ab assay. Moreover, the modified anti-AChR Ab assay, differently from the conventional assay, allowed us to reveal anti-AChR Ab concentration differences among different clinical grades of MG.


Key Words: indexing terms: myasthenia gravis • radioreceptor assay • intermethod comparison






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Copyright © 1997 by the American Association for Clinical Chemistry.