Clinical Chemistry
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Clinical Chemistry 43: 1003-1009, 1997;
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(Clinical Chemistry. 1997;43:1003-1009.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Highly sensitive gas chromatographic analysis of ethanol in whole blood, serum, urine, and fecal supernatants by the direct injection method

Albert Tangerman

Department of Gastroenterology and Hepatology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands.
Address for correspondence: Laboratory of Gastroenterology and Hepatology, University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands. Fax 0031-24-3540103; e-mail A.Tangerman{at}gastro.azn.nl

A highly sensitive, reproducible, and rapid gas chromatographic method for ethanol determination in various biological specimens (human whole blood, serum, urine, and fecal supernatants) was developed. The method involves direct injection of the biological specimen into the gas chromatograph, without any pretreatment. Contamination of the gas chromatographic column with nonvolatile material was prevented by the use of a glass liner in the injector. This liner, which acted as a precolumn, was partly filled with small glass beads. Injection was performed in between the glass beads. More than 50 injections of the various biological specimens could be done before the liner had to be replaced by a new one. This injection technique between glass beads allows direct injection of large sample volumes up to 10 µL without disturbing the gas chromatographic separation. Injection of these large sample volumes made the method very sensitive. The detection limit for ethanol amounted to 0.1 mg/L (2 µmol/L) when using an injection volume of 5 µL. Attention has also been paid to simultaneously monitoring ethanol, methanol, acetaldehyde, and acetone in blood and urine of control subjects.


Key Words: indexing terms: alcohol • acetaldehyde • acetone • methanol • propanol • butanol.




The following articles in journals at HighWire Press have cited this article:


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An isotopic method for quantifying sweeteners derived from corn and sugar cane
Am. J. Clinical Nutrition, December 1, 2006; 84(6): 1380 - 1384.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.