Clinical Chemistry
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Clinical Chemistry 43: 1010-1015, 1997;
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(Clinical Chemistry. 1997;43:1010-1015.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Determination of D-lactate by enzymatic methods in biological fluids: study of interferences

Ramon Martía, Encarna Varela, Rosa M. Segura, José Alegre1, José M. Suriñach1 and Carles Pascual

1 Serveis de Bioquímica i Medicina Interna, Hospital General Universitari "Vall d'Hebron," P. Vall d'Hebron 119–129, 08035-Barcelona, Spain.
a Author for correspondence. Fax +34-3-4280443.

Analysis of nondeproteinized samples with an enzymatic method to determine D-lactate indicated interferences. The presence of L-lactate dehydrogenase (LD) and L-lactate in the sample led to underestimation of D-lactate content when a sample blank was processed and overestimation when it was omitted. We proved that this interference is not due to lack of D-LD stereospecificity. Moreover, assessment of D-LD and L-LD KM for NAD+ allowed us to rule out the different affinities for this coenzyme as a cause of the interference. Our results underline the importance of deproteinizing samples for D-lactate analysis when enzymatic methods are used. The ultrafiltration procedure we propose is convenient and shows acceptable mean recovery (108%) and good imprecision (within-run CV = 4.2% and 3.0% for D-lactate at 31 and 107 µmol/L, respectively; between-run CVs were 7.3% at 49 µmol/L D-lactate and 3.1% at 115 µmol/L D-lactate).


Key Words: indexing terms: interference, source of • bacterial infections • D-lactate acidosis • ultrafiltration • sample preparation




The following articles in journals at HighWire Press have cited this article:


Home page
 Ann Clin BiochemHome page
D. J. Herrera, K. Morris, C. Johnston, and P. Griffiths
Automated assay for plasma D-lactate by enzymatic spectrophotometric analysis with sample blank correction
Ann Clin Biochem, March 1, 2008; 45(2): 177 - 183.
[Abstract] [Full Text] [PDF]


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Copyright © 1997 by the American Association for Clinical Chemistry.