Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 43: 1029-1032, 1997;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Broussard, L. A.
Right arrow Articles by Wimbish, G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Broussard, L. A.
Right arrow Articles by Wimbish, G. H.
Related Collections
Right arrow Drug Monitoring and Toxicology
(Clinical Chemistry. 1997;43:1029-1032.)
© 1997 American Association for Clinical Chemistry, Inc.

Articles

Simultaneous identification and quantitation of codeine, morphine, hydrocodone, and hydromorphone in urine as trimethylsilyl and oxime derivatives by gas chromatography–mass spectrometry

Larry A. Broussard1,a, Lance C. Presley2, Thomas Pittman3, Randy Clouette4 and Gary H. Wimbish4

1 Department of Medical Technology, LSU Medical Center, New Orleans, LA 70112-2262.

2 LabCorp, Memphis, TN 38118.

3 TF Puckett Laboratory, Hattiesburg, MS 39402.

4 Harrison Laboratories, Midland, TX 79706.
a Author for correspondence. Fax 504-568-6761; e-mail lbrous{at}lsumc.edu

Following enzymatic hydrolysis of urine, a gas chromatography–mass spectrometry method for the simultaneous determination of codeine, morphine, hydrocodone, and hydromorphone uses hydroxylamine to form oxime derivatives of the keto-opiates (i.e., hydrocodone, hydromorphone, oxycodone, and oxymorphone). These trimethylsilyl-derivatized forms no longer interfere with the detection and quantitation of codeine and morphine. Samples are extracted on solid-phase columns and quantitated by deuterated internal calibrations of each analyte with selected ion monitoring. Codeine, morphine, hydrocodone, and hydromorphone are completely separated, allowing simultaneous quantitation without interference and a chromatographic analysis time <9 min.


Key Words: indexing terms: abused drugs • drug monitoring • opiates • forensic toxicology




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
L. A. Broussard, L. C. Presley, M. Tanous, and C. Queen
Improved Gas Chromatography-Mass Spectrometry Method for Simultaneous Identification and Quantification of Opiates in Urine as Propionyl and Oxime Derivatives
Clin. Chem., January 1, 2001; 47(1): 127 - 129.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1997 by the American Association for Clinical Chemistry.